Piroozmand Ahmad, Soltani Babak, Razavizadeh Mohsen, Matini Amir Hasan, Gilasi Hamid Reza, Zavareh Abbas Nassaji, Soltani Siamak
Ph.D. of Virology, Associate Professor, Department of Microbiology, Faculty of Medicine, Kashan University of Medical Sciences, Kashan, Iran.
M.D., Pediatric Infectious Diseases Specialist, Associate Professor, Department of Pediatrics, Faculty of Medicine, Kashan University of Medical Sciences, Kashan, Iran.
Electron Physician. 2017 Dec 25;9(12):6129-6134. doi: 10.19082/6129. eCollection 2017 Dec.
Helicobacter pylori () is an important and common contagious human pathogen which may cause peptic ulcer and also gastric cancer. The definite diagnosis of it is made through invasive tests. Recently, non-invasive tests including serologic tests of serum and saliva have been conducted for diagnosis of infection. In this research, the diagnostic values of serum and salivary serology were compared together to use salivary anti- test as an alternative method in the future.
During this prospective case-control study on patients who were candidates for endoscopy and gastric biopsy from March 2015 to April 2016 in Shahid Beheshti hospital, Kashan, Iran, serum and salivary samples were obtained for measurement of Immunoglobulin G (IgG) antibody levels against by enzyme-linked immunosorbent assay (ELISA). Histopathology was the gold standard test. Statistical analysis was performed by SPSS software version 16. Statistical tests included Kolmogorov-Smirnov, independent-samples t-test, Chi-square, Mann-Whitney U, Kruskal-Wallis, McNemar and correlation.
Of 123 patients, sixty-one patients (49.6%) were -positive according to histology. The median levels of anti- antibodies in serum (p<0.001) and saliva (p<0.001) of -positive cases were significantly higher than -negative cases. Sensitivity, specificity, positive likelihood ratio, negative likelihood ratio and accuracy of serologic tests in serum were 75%, 79%, 3.5, 0.3, 77% and for saliva were 85%, 82%, 4.7, 0.18, 84% respectively.
Diagnostic values of salivary ELISA are comparable to serum ELISA and can be used as an alternative modality for diagnosis of infection.
幽门螺杆菌（Hp）是一种重要且常见的人类传染性病原体，可导致消化性溃疡以及胃癌。其确诊需通过侵入性检测。近来，包括血清和唾液血清学检测在内的非侵入性检测已用于Hp感染的诊断。在本研究中，对血清和唾液血清学的诊断价值进行比较，以便未来将唾液抗Hp检测用作替代方法。
在2015年3月至2016年4月于伊朗卡尚的沙希德·贝赫什提医院对拟进行内镜检查和胃活检的患者开展的这项前瞻性病例对照研究中，采集血清和唾液样本，通过酶联免疫吸附测定（ELISA）检测抗Hp免疫球蛋白G（IgG）抗体水平。组织病理学检查为金标准检测。采用SPSS 16版软件进行统计分析。统计检验包括柯尔莫哥洛夫-斯米尔诺夫检验、独立样本t检验、卡方检验、曼-惠特尼U检验、克鲁斯卡尔-沃利斯检验、 McNemar检验和相关性分析。
123例患者中，根据组织学检查有61例患者（49.6%）为Hp阳性。Hp阳性病例血清（p<0.001）和唾液（p<0.001）中抗Hp抗体的中位数水平显著高于Hp阴性病例。血清学检测在血清中的敏感性、特异性、阳性似然比、阴性似然比和准确性分别为75%、79%、3.5、0.3、77%，在唾液中的分别为85%、82%、4.7、0.18、84%。
唾液ELISA的诊断价值与血清ELISA相当，可作为诊断Hp感染的替代方法。
