Key Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of Education, Key Laboratory of Analytical Science and Technology of Hebei Province, College of Chemistry and Environmental Science, Hebei University, Baoding 071002, Hebei, P. R. China.
Analyst. 2018 Apr 16;143(8):1758-1774. doi: 10.1039/C7AN02001E.
The development of simple, robust, and reliable microRNA (miRNA) detection methods is of great significance in the studies of the biological function of miRNAs, molecular diagnostics, treatment of diseases, and targeted drugs. In recent years, with the increasing development of miRNA research, lots of novel approaches were developed for the detection of miRNA in terms of sensitivity, specificity, multiplicity, in situ imaging, etc. In particular, nucleic acid amplification-based methods and many detection techniques such as droplet digital PCR (ddPCR), electrochemiluminescence (ECL), surface-enhanced Raman spectroscopy (SERS), and mass spectrometry (MS) have been employed widely for the highly sensitive detection of miRNA. New progress in miRNA detection has accelerated miRNA functional research and clinical diagnostics. In this review, we summarize the recent progress in the development of miRNA detection methods and new applications. This review will provide guidelines for the development of more advanced miRNA detection methods with high sensitivity and specificity, and applicability to biochemical research, disease diagnosis and therapy.
开发简单、稳健、可靠的 microRNA（miRNA）检测方法对于 miRNA 的生物学功能研究、分子诊断、疾病治疗和靶向药物具有重要意义。近年来，随着 miRNA 研究的不断发展，针对 miRNA 的检测已经出现了许多新型方法，这些方法在灵敏度、特异性、多样性、原位成像等方面都有显著提高。特别是基于核酸扩增的方法以及许多检测技术，如微滴式数字 PCR（ddPCR）、电化学发光（ECL）、表面增强拉曼光谱（SERS）和质谱（MS），已被广泛用于 miRNA 的高灵敏度检测。miRNA 检测的新进展加速了 miRNA 功能研究和临床诊断。本综述总结了 miRNA 检测方法的最新进展和新应用。这将为开发具有高灵敏度和特异性以及适用于生化研究、疾病诊断和治疗的更先进的 miRNA 检测方法提供指导。
