Key Laboratory of Horticultural Plant Biology, Ministry of Education, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, China.
Hubei Key Laboratory of Agricultural Bioinformatics, College of Informatics, Huazhong Agricultural University, Wuhan 430070, China.
Int J Mol Sci. 2018 Mar 21;19(4):922. doi: 10.3390/ijms19040922.
Cytoplasmic male sterility (CMS) is universally utilized in cruciferous vegetables. However, the Chinese cabbage CMS lines, obtained by interspecific hybridization and multiple backcrosses of the () CMS line and Chinese cabbage, show obvious leaf etiolation, and the molecular mechanism of etiolation remains elusive. Here, the ultrastructural and phenotypic features of leaves from the Chinese cabbage CMS line 1409A and maintainer line 1409B are analyzed. The results show that chloroplasts of 1409A exhibit abnormal morphology and distribution. Next, RNA-sequencing (RNA-Seq) is used to identify 485 differentially expressed genes (DEGs) between 1409A and 1409B, and 189 up-regulated genes and 296 down-regulated genes are found. Genes that affect chloroplasts development, such as and , and chlorophyll biosynthesis, such as , are included in the down-regulated DEGs. Quantitative real-time PCR (qRT-PCR) analysis validate that the expression levels of these genes are significantly lower in 1409A than in 1409B. Taken together, these results demonstrate that leaf etiolation is markedly affected by chloroplast development and pigment biosynthesis. This study provides an effective foundation for research on the molecular mechanisms of leaf etiolation of the CMS line in Chinese cabbage ( L. ssp. ).
细胞质雄性不育（CMS）普遍应用于十字花科蔬菜。然而，通过种间杂交和多次回交（）CMS 系和白菜，获得的白菜 CMS 系表现出明显的叶片黄化，黄化的分子机制仍不清楚。在这里，分析了白菜 CMS 系 1409A 和保持系 1409B 叶片的超微结构和表型特征。结果表明，1409A 的叶绿体表现出异常的形态和分布。接下来，使用 RNA 测序（RNA-Seq）鉴定 1409A 和 1409B 之间的 485 个差异表达基因（DEG），发现 189 个上调基因和 296 个下调基因。包括影响叶绿体发育的基因如和，以及叶绿素生物合成的基因如，都包含在下调的 DEG 中。定量实时 PCR（qRT-PCR）分析验证了这些基因在 1409A 中的表达水平明显低于 1409B。综上所述，这些结果表明，叶绿体发育和色素生物合成明显影响叶片黄化。本研究为白菜 CMS 系叶片黄化的分子机制研究提供了有效的基础（ L. ssp. ）。
