Zhou Fuhui, Liu Yang, Feng Xin, Zhang Yuting, Zhu Pengfang
College of Forestry, Shenyang Agricultural University, Shenyang, China.
Key Laboratory of Forest Tree Genetics, Breeding and Cultivation of Liaoning Province, Shenyang, China.
Front Plant Sci. 2022 Apr 27;13:769121. doi: 10.3389/fpls.2022.769121. eCollection 2022.
Leaf color is a crucial agronomic trait in ornamental kale. However, the molecular mechanism regulating leaf pigmentation patterns in green and white ornamental kale is not completely understood. To address this, we performed transcriptome and pigment content analyses of green and white kale leaf tissues. A total of 5,404 and 3,605 different expressed genes (DEGs) were identified in the green vs. white leaf and the green margin vs. white center samples. Kyoto Encyclopedia of Genes and Genome (KEGG) pathway enrichment analysis showed that 24 and 15 common DEGs in two pairwise comparisons were involved in chlorophyll metabolism and carotenoid biosynthesis, respectively. Seventeen genes related to chlorophyll biosynthesis were significantly upregulated in green leaf tissue, especially and . Of the 15 carotenoid biosynthesis genes, all except and were lower expressed in white leaf tissue. Green leaf tissue exhibited higher levels of chlorophyll and carotenoids than white leaf tissue. In addition, the DEGs involved in photosystem and chlorophyll-binding proteins had higher expression in green leaf tissue. The , , , and may be key genes of photosynthesis and chloroplast formation. These results demonstrated that green and white coloration in ornamental kale leaves was caused by the combined effects of chlorophyll and carotenoid biosynthesis, chloroplast development, as well as photosynthesis. These findings enhance our understanding of the molecular mechanisms underlying leaf color development in ornamental kale.
叶色是观赏羽衣甘蓝的一个关键农艺性状。然而，调控绿色和白色观赏羽衣甘蓝叶片色素沉着模式的分子机制尚未完全明确。为解决这一问题，我们对绿色和白色羽衣甘蓝叶片组织进行了转录组和色素含量分析。在绿叶与白叶以及绿边与白心样本中分别鉴定出5404个和3605个差异表达基因（DEG）。京都基因与基因组百科全书（KEGG）通路富集分析表明，在两个成对比较中的24个和15个常见DEG分别参与叶绿素代谢和类胡萝卜素生物合成。与叶绿素生物合成相关的17个基因在绿叶组织中显著上调，尤其是 和 。在15个类胡萝卜素生物合成基因中，除 和 外，其余在白叶组织中表达较低。绿叶组织中的叶绿素和类胡萝卜素水平高于白叶组织。此外，参与光系统和叶绿素结合蛋白的DEG在绿叶组织中具有更高的表达。 、 、 和 可能是光合作用和叶绿体形成的关键基因。这些结果表明，观赏羽衣甘蓝叶片的绿色和白色是由叶绿素和类胡萝卜素生物合成、叶绿体发育以及光合作用的综合作用引起的。这些发现加深了我们对观赏羽衣甘蓝叶片颜色发育潜在分子机制的理解。
