Kumar Sunjeet, Huang Xinfang, Li Gaojie, Ji Qun, Zhou Kai, Zhu Guopeng, Ke Weidong, Hou Hongwei, Zhu Honglian, Yang Jingjing
The State Key Laboratory of Freshwater Ecology and Biotechnology, The Key Laboratory of Aquatic Biodiversity and Conservation of Chinese Academy of Sciences, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China.
University of Chinese Academy of Sciences, Beijing 100049, China.
Plants (Basel). 2021 Nov 17;10(11):2484. doi: 10.3390/plants10112484.
In the agricultural field, blanching is a technique used to obtain tender, sweet, and delicious water dropwort stems by blocking sunlight. The physiological and nutritional parameters of blanched water dropwort have been previously investigated. However, the molecular mechanism of blanching remains unclear. In the present study, we investigated transcriptomic variations for different blanching periods in the stem of water dropwort (pre, mid, post-blanching, and control). The results showed that many genes in pathways, such as photosynthesis, carbon fixation, and phytohormone signal transduction as well as transcription factors (TFs) were significantly dysregulated. Blanched stems of water dropwort showed the higher number of downregulated genes in pathways, such as photosynthesis, antenna protein, carbon fixation in photosynthetic organisms, and porphyrin and chlorophyll metabolism, which ultimately affect the photosynthesis in water dropwort. The genes of hormone signal transduction pathways (ethylene, jasmonic acid, brassinosteroid, and indole-3-acetic acid) showed upregulation in the post-blanched water dropwort plants. Overall, a higher number of genes coding for TFs, such as ERF, BHLH, MYB, zinc-finger, bZIP, and WRKY were overexpressed in blanched samples in comparison with the control. These genes and pathways participate in inducing the length, developmental processes, pale color, and stress tolerance of the blanched stem. Overall, the genes responsive to blanching, which were identified in this study, provide an effective foundation for further studies on the molecular mechanisms of blanching and photosynthesis regulations in water dropwort and other species.
在农业领域，软化栽培是一种通过遮光来获取鲜嫩、甜美且美味的水芹茎的技术。此前已经对软化栽培水芹的生理和营养参数进行了研究。然而，软化栽培的分子机制仍不清楚。在本研究中，我们调查了水芹茎在不同软化栽培时期（前期、中期、后期和对照）的转录组变化。结果表明，光合作用、碳固定、植物激素信号转导以及转录因子等途径中的许多基因都发生了显著的失调。软化栽培的水芹茎在光合作用、天线蛋白、光合生物中的碳固定以及卟啉和叶绿素代谢等途径中显示出更多下调的基因，这最终影响了水芹的光合作用。激素信号转导途径（乙烯、茉莉酸、油菜素内酯和吲哚 - 3 - 乙酸）的基因在软化栽培后期的水芹植株中上调。总体而言，与对照相比，在软化栽培样本中，编码转录因子如ERF、BHLH、MYB、锌指、bZIP和WRKY的基因有更多的过表达。这些基因和途径参与诱导软化栽培茎的长度、发育过程、淡色和胁迫耐受性。总体而言，本研究中鉴定出的对软化栽培有响应的基因，为进一步研究水芹及其他物种中软化栽培和光合作用调控的分子机制提供了有效的基础。
