Grand R J, Rowe M, Byrd P J, Gallimore P H
Int J Cancer. 1987 Aug 15;40(2):213-9. doi: 10.1002/ijc.2910400215.
The level of expression of the class-I major histocompatibility (MHC) antigen was determined in a series of human embryo cell lines transformed with either adenovirus 12 (Ad 12) early region I (EI) or adenovirus 5 (Ad 5) EI DNA or with combinations of Ad 12 early region IA (EIA) or Ad 2 EIA with activated N-ras DNA. MHC class-I antigen expression was greatly reduced in all Ad 12 transformants, compared to primary cells. Expression was also reduced in the Ad 5 cell lines transformed with EIA and EIB DNA, but levels were near normal in those lines with only EIA DNA present. Amounts of MHC class-I antigen on the cell surface, as determined by RIA and FACS analysis, generally reflected total cellular levels as determined by Western blotting. Expression of beta 2 microglobulin was also much reduced in those cell lines with low levels of MHC class-I antigen. The treatment of primary cells and all the transformants with human gamma-interferon resulted in increased expression of HLA on the cell surface. Infection of primary human cells with Ad 12 or with a series of Ad 12 mutants did not have any effect on the MHC class-I antigens present.
在一系列用腺病毒12（Ad 12）早期区域I（EI）或腺病毒5（Ad 5）EI DNA或用Ad 12早期区域IA（EIA）或Ad 2 EIA与活化的N-ras DNA的组合转化的人胚胎细胞系中，测定了I类主要组织相容性（MHC）抗原的表达水平。与原代细胞相比，所有Ad 12转化体中MHC I类抗原表达均大幅降低。在用EIA和EIB DNA转化的Ad 5细胞系中表达也降低，但在仅存在EIA DNA的那些细胞系中水平接近正常。通过放射免疫分析（RIA）和荧光激活细胞分选（FACS）分析测定的细胞表面MHC I类抗原量，通常反映了通过蛋白质印迹法测定的总细胞水平。在那些MHC I类抗原水平低的细胞系中，β2微球蛋白的表达也大大降低。用人γ干扰素处理原代细胞和所有转化体导致细胞表面HLA表达增加。用Ad 12或一系列Ad 12突变体感染原代人细胞对存在的MHC I类抗原没有任何影响。
