Guelph Research and Development Centre, Agriculture and Agri-Food Canada, Guelph, Ontario, N1G 5C9, Canada.
Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, Hunan, 410125, China.
Sci Rep. 2018 Mar 21;8(1):4940. doi: 10.1038/s41598-018-22565-6.
Butyrate can modulate the immune response and energy expenditure of animals and enhance intestinal health. The present study investigated changes in the intestinal microbiota composition and serum metabolites of young broilers in response to 3,000 ppm butyrate in the form of butyrate glycerides (BG) via pyrosequencing of bacterial 16S rRNA genes and nuclear magnetic resonance (NMR). The dietary treatment did not affect the alpha diversity of intestinal microbiota, but altered its composition. Thirty-nine key operational taxonomic units (OTUs) in differentiating cecal microbiota community structures between BG treated and untreated chickens were also identified. Bifidobacterium was, in particular, affected by the dietary treatment significantly, showing an increase in not only the abundance (approximately 3 fold, P ≤ 0.05) but also the species diversity. The (NMR)-based analysis revealed an increase in serum concentrations of alanine, low-density and very low-density lipoproteins, and lipids (P ≤ 0.05) by BG. More interestingly, the dietary treatment also boosted (P ≤ 0.05) serum concentrations of bacterial metabolites, including choline, glycerophosphorylcholine, dimethylamine, trimethylamine, trimethylamine-N-oxide, lactate, and succinate. In conclusion, the data suggest the modulation of intestinal microbiota and serum metabolites by BG dietary treatment and potential contribution of intestinal bacteria to lipid metabolism/energy homeostasis in broilers.
丁酸盐可以调节动物的免疫反应和能量消耗，增强肠道健康。本研究通过细菌 16S rRNA 基因焦磷酸测序和核磁共振（NMR）技术，研究了以丁酸盐甘油酯（BG）形式添加 3000ppm 丁酸盐对肉鸡肠道微生物群落组成和血清代谢物的影响。日粮处理对肠道微生物群落的 alpha 多样性没有影响，但改变了其组成。还确定了 39 个区分 BG 处理和未处理鸡盲肠微生物群落结构的关键操作分类单元（OTU）。双歧杆菌尤其受到日粮处理的显著影响，不仅丰度增加（约 3 倍，P≤0.05），而且物种多样性也增加。基于（NMR）的分析表明，BG 增加了血清中丙氨酸、低密度和极低密度脂蛋白以及脂质的浓度（P≤0.05）。更有趣的是，日粮处理还增加了血清中细菌代谢物的浓度，包括胆碱、甘油磷酸胆碱、二甲胺、三甲胺、三甲胺-N-氧化物、乳酸和琥珀酸（P≤0.05）。总之，这些数据表明 BG 日粮处理可以调节肠道微生物群落和血清代谢物，并可能促进肠道细菌对肉鸡脂质代谢/能量平衡的贡献。
