Tie Kunyuan, Yuan Yuyu, Yan Shiqing, Yu Xi, Zhang Qiuyang, Xu Huihui, Zhang Yang, Gu Jingmin, Sun Changjiang, Lei Liancheng, Han Wenyu, Feng Xin
College of Veterinary Medicine, Jilin University, No. 5333 Xi'an Road, Changchun, 130062, Jilin, People's Republic of China.
College of Life Science and Technology, Dalian University of Technology, No. 2 Lingshui Road, Dalian, 116024, Liaoning, People's Republic of China.
Virus Genes. 2018 Jun;54(3):446-456. doi: 10.1007/s11262-018-1549-0. Epub 2018 Mar 21.
Salmonella pullorum is the major pathogen that is harmful to the poultry industry in developing countries, and the treatment of chicken diarrhea caused by S. pullorum has become increasingly difficult. In this study, a virulent bacteriophage YSP2, which was able to specifically infect Salmonella, was isolated and characterized. Phage YSP2 was classified in the Siphoviridae family and had a short latent period of 10 min. No bacterial virulence- or lysogenesis-related ORF is present in the YSP2 genome, making it eligible for use in phage therapy. Experiments in vivo investigated the potential use of phages as a therapy against diarrhea in chickens caused by S. pullorum in a chicken diarrhea model, demonstrating that a single oral administration of YSP2 (1 × 10 PFU/mL, 80 μL/chicken) 2 h after S. pullorum oral administration at a double median lethal dose was sufficient to protect chickens against diarrhea. Gross inspection showed that YSP2 can effectively reduce organ damage and significantly relieve hemorrhage in the intestine and liver tissue. Moreover, YSP2 can maintain a high curative effect when diluted to 10 PFU/mL. In light of its therapeutic effect on chicken diarrhea, YSP2 may serve as an alternative treatment strategy for infections caused by S. pullorum.
鸡白痢沙门氏菌是发展中国家对家禽业有害的主要病原体，由鸡白痢沙门氏菌引起的鸡腹泻的治疗变得越来越困难。在本研究中，分离并鉴定了一种能够特异性感染沙门氏菌的烈性噬菌体YSP2。噬菌体YSP2属于长尾噬菌体科，潜伏期短，为10分钟。YSP2基因组中不存在与细菌毒力或溶原性相关的开放阅读框，这使其有资格用于噬菌体治疗。体内实验在鸡腹泻模型中研究了噬菌体作为治疗鸡白痢沙门氏菌引起的腹泻的潜在用途，结果表明，在以双倍半数致死剂量口服鸡白痢沙门氏菌2小时后，单次口服YSP2（1×10⁹ PFU/mL，80 μL/只鸡）足以保护鸡免于腹泻。大体检查表明，YSP2可有效减轻器官损伤，并显著缓解肠道和肝脏组织的出血。此外，YSP2稀释至10⁶ PFU/mL时仍可保持较高的疗效。鉴于其对鸡腹泻的治疗效果，YSP2可能成为治疗鸡白痢沙门氏菌感染的替代治疗策略。
