利用直流视网膜电图分析小鼠模型中的视网膜色素上皮功能

Use of Direct Current Electroretinography for Analysis of Retinal Pigment Epithelium Function in Mouse Models.

作者信息

Yu Minzhong, Peachey Neal S

机构信息

Department of Ophthalmic Research, Cole Eye Institute, Cleveland Clinic Foundation, Cleveland, OH, USA.

Department of Ophthalmology, Cleveland Clinic Lerner College of Medicine of Case Western Reserve University, Cleveland, OH, USA.

出版信息

Methods Mol Biol. 2018;1753:103-113. doi: 10.1007/978-1-4939-7720-8_7.

DOI:10.1007/978-1-4939-7720-8_7

PMID:29564784

Abstract

A monolayer of pigmented epithelial cells, the retinal pigment epithelium (RPE), supports photoreceptor function in many ways. Consistent with these roles, RPE dysfunction underlies a number of hereditary retinal disorders. To monitor RPE function in vivo models for these conditions, we adapted an electroretinographic (ERG) technique based on direct current amplification (DC-ERG). This chapter describes the main features of this approach and its application to mouse models involving the RPE.

摘要

一层色素上皮细胞，即视网膜色素上皮（RPE），在许多方面支持光感受器功能。与这些作用相一致，RPE功能障碍是许多遗传性视网膜疾病的基础。为了在这些病症的体内模型中监测RPE功能，我们采用了一种基于直流放大的视网膜电图（ERG）技术（直流ERG）。本章描述了这种方法的主要特点及其在涉及RPE的小鼠模型中的应用。