a Department of Prosthetic Dental Sciences, College of Dentistry , King Saud University , Riyadh , Saudi Arabia.
b Ministry of Interior Medical Services , Security Forces Hospital Program , Riyadh , Saudi Arabia.
Inhal Toxicol. 2018 Feb;30(2):72-77. doi: 10.1080/08958378.2018.1449273. Epub 2018 Mar 22.
It is hypothesized that levels of matrix metalloproteinase (MMP)-8 and MMP-9 are significantly higher in the peri-implant sulcular fluid (PISF) of waterpipe-smokers (WS) compared with never-smokers with peri-implantitis. The aim of the present convenience sample case-control study was to compare the levels of MMP-8 and MMP-9 in the PISF of WS and never-smokers with peri-implantitis.
Individuals smoking waterpipe (Group 1) and never-smokers (Group 2) were included. Demographic data was collected using a questionnaire. Peri-implant probing depth (PPD) was measured and crestal bone loss (CBL) was measured on digital bitewing radiographs. PISF samples were collected using paper strips and the collected PISF volume was determined. Levels of MMP-8 and MMP-9 were measured using enzyme-linked immunosorbent assay. Study sample-size was estimated and statistical analysis was performed. p values < .05 were considered statistically significant.
Sixty-six individuals (33 individuals in Group 1 and 33 in Group 2) were included. In Groups 1 and 2, 41 and 44 implants, respectively were placed. The mean total PPD (p < .001) and peri-implant CBL (p < .001) was statistically significantly higher around implants affected by peri-implantitis in Group 1 compared with Group 2. The PISF volume (p < .05) collected and levels of MMP-8 (p < .01) and MMP-9 (p < .01) were statistically significantly higher among individuals in Group 1 compared with Group 2.
PISF levels of MMP-8 and MMP-9 are significantly higher among WS compared with never-smokers with peri-implantitis.
假设与患有种植体周围炎的从不吸烟者相比，水烟吸烟者（WS）的种植体周围龈沟液（PISF）中基质金属蛋白酶（MMP）-8 和 MMP-9 的水平显著更高。本便利样本病例对照研究的目的是比较 WS 和从不吸烟者的 PISF 中 MMP-8 和 MMP-9 的水平。
纳入了吸烟水烟的个体（第 1 组）和从不吸烟的个体（第 2 组）。使用问卷收集人口统计学数据。测量种植体探诊深度（PPD）和牙槽嵴骨丧失（CBL）。使用纸条收集 PISF 样本，并确定收集的 PISF 量。使用酶联免疫吸附测定法测量 MMP-8 和 MMP-9 的水平。估计了研究样本量并进行了统计分析。p 值<.05 被认为具有统计学意义。
纳入了 66 名个体（第 1 组 33 名，第 2 组 33 名）。第 1 组和第 2 组分别植入了 41 个和 44 个种植体。第 1 组中受种植体周围炎影响的种植体周围总 PPD（p<.001）和种植体周围 CBL（p<.001）明显高于第 2 组。与第 2 组相比，第 1 组收集的 PISF 量（p<.05）、MMP-8 水平（p<.01）和 MMP-9 水平（p<.01）明显更高。
与患有种植体周围炎的从不吸烟者相比，WS 的 PISF 中 MMP-8 和 MMP-9 的水平明显更高。
