Cancer Research Institute, Tomsk National Research Medical Center, Tomsk 634050, Russia.
Siberian State Medical University, Tomsk 634050, Russia.
Molecules. 2018 Mar 22;23(4):727. doi: 10.3390/molecules23040727.
The biological properties of circulating tumor cells (CTCs), and their dynamics during neoadjuvant chemotherapy are important, both for disease progression prediction and therapeutic target determination, with the aim of preventing disease progression. The aim of our study was to estimate of different CTC subsets in breast cancer during the NACT (neoadjuvant chemotherapy). The prospective study includes 27 patients with invasive breast cancer, T2-4N0-3M0, aged 32 to 60 years. Venous heparinized blood samples, taken before and after biopsy, after each courses of chemotherapy (on days 3-7), and before surgical intervention, served as the material for this study. Different subsets of circulating tumor cells were determined on the basis of the expression of EpCAM, CD45, CD44, CD24, and N-Cadherin using flow cytometry. As the result of this study, it has been observed that significant changes in the quantity of the different subsets of circulating tumor cells in patients' blood were observed after carrying out the 3rd course of NACT. NACT causes significant changes in the quantity of six CTC subsets, with various combinations of stemness and epithelial-mesenchymal transition (EMT) properties.
循环肿瘤细胞（CTC）的生物学特性及其在新辅助化疗期间的动态变化对于疾病进展预测和治疗靶点的确定非常重要，目的是防止疾病进展。我们的研究目的是评估新辅助化疗期间乳腺癌中不同的 CTC 亚群。这项前瞻性研究包括 27 名浸润性乳腺癌患者，年龄 32 至 60 岁，T2-4N0-3M0。静脉肝素化血样取自活检前、每次化疗后（第 3-7 天）和手术干预前，作为本研究的材料。使用流式细胞术根据 EpCAM、CD45、CD44、CD24 和 N-钙黏蛋白的表达来确定循环肿瘤细胞的不同亚群。本研究结果表明，在进行第 3 个疗程的新辅助化疗后，患者血液中不同循环肿瘤细胞亚群的数量发生了显著变化。新辅助化疗导致 6 种 CTC 亚群的数量发生显著变化，具有不同的干性和上皮-间充质转化（EMT）特性组合。
