Laboratory of Stem Cells and Tissue Engineering, Department of Histology and Embryology, Chongqing Medical University, Chongqing 400016, China; The First Affiliated Hospital of Chongqing Medical University, Chongqing, China.
Laboratory of Stem Cells and Tissue Engineering, Department of Histology and Embryology, Chongqing Medical University, Chongqing 400016, China.
Int Immunopharmacol. 2018 May;58:94-102. doi: 10.1016/j.intimp.2018.03.017. Epub 2018 Mar 19.
Physiological aging is associated with a range of medical problems. However, the treatment of aging-associated diseases and prolonging human life are vital to our current aging societies. Panax ginseng, a traditional Chinese medicine, has been shown to have anti-oxidative and anti-aging effects. In the current study, aging rats induced by d-galactose were administered ginsenoside Rg1, then splenocytes and thymocytes were extracted and changes in activity were detected. The results demonstrated that compared with the d-gal group, the level of advanced glycation end products (AGE), the ratio of splenocytes and thymocytes in G0 phase (%), and apoptosis (%) of splenocytes and thymocytes, the ratio (%) of SA-gal positive splenocytes and thymocytes, the content of reactive oxygen species (ROS) and malondialdehyde (MDA), the ratio of glutathione (GSH) to oxidized glutathione (GSSG) and senescence-associated protein expression were significantly decreased and the index of the spleen and thymus, the proportion of white pulp in the spleen, the proportion of cortex in the thymus, the content of interleukin-2 (IL-2), interleukin-6 (IL-6), granulocyte-macrophage colony stimulating factor (GM-CSF), tumor necrosis factor-α (TNF-α), the activities of superoxide dismutase (SOD), and the proliferative capacity of splenocytes and thymocytes were increased in the Rg1+ d-gal group. These findings demonstrated that ginsenoside Rg1 may antagonize spleen and thymus damage in d-galactose-induced aging rats by alleviating oxidative stress injury and down-regulating the expression of senescence-associated protein.
生理衰老与一系列医学问题有关。然而，治疗与衰老相关的疾病和延长人类寿命对我们当前的老龄化社会至关重要。人参是一种传统的中药，已被证明具有抗氧化和抗衰老作用。在目前的研究中，用 D-半乳糖诱导衰老大鼠，然后提取脾细胞和胸腺细胞并检测其活性变化。结果表明，与 D-半乳糖组相比，晚期糖基化终产物（AGE）水平、G0 期脾细胞和胸腺细胞的比例（%）、脾细胞和胸腺细胞凋亡（%）、SA-gal 阳性脾细胞和胸腺细胞的比例（%）、活性氧（ROS）和丙二醛（MDA）含量、谷胱甘肽（GSH）与氧化型谷胱甘肽（GSSG）的比值以及衰老相关蛋白的表达水平均显著降低，脾和胸腺指数、脾白髓比例、胸腺皮质比例、白细胞介素-2（IL-2）、白细胞介素-6（IL-6）、粒细胞-巨噬细胞集落刺激因子（GM-CSF）、肿瘤坏死因子-α（TNF-α）的含量、超氧化物歧化酶（SOD）活性以及脾细胞和胸腺细胞的增殖能力均增加。这些发现表明，人参皂苷 Rg1 可能通过减轻氧化应激损伤和下调衰老相关蛋白的表达来拮抗 D-半乳糖诱导的衰老大鼠的脾和胸腺损伤。
