Kumthip Kattareeya, Khamrin Pattara, Saikruang Wilaiporn, Kongkaew Aphisek, Vachirachewin Ratchaya, Ushijima Hiroshi, Maneekarn Niwat
Department of Microbiology, Faculty of Medicine, Chiang Mai University, Chiang Mai, 50200, Thailand.
Center of Excellence in Emerging and Re-emerging Diarrheal Viruses, Chiang Mai University, Chiang Mai, Thailand.
Arch Virol. 2018 Jul;163(7):1823-1829. doi: 10.1007/s00705-018-3806-x. Epub 2018 Mar 22.
Porcine astrovirus (PAstV) is widely distributed and highly prevalent among pigs, nevertheless its clinical significance remains unclear as it can be detected in both diarrheic and in healthy pigs. Information about the prevalence, clinical significance and molecular characterization of PAstV in Thailand is not available. This study investigated the prevalence of PAstV in 488 fecal samples collected from piglets with and without diarrhea in 28 pig farms in northern and central parts of Thailand using RT-PCR. The overall prevalence of PAstV infection was 6.5% (32/488), of which 21/251 (8.4%) were in diarrheic and 11/237 (4.6%) were in healthy pigs. Of 32 positive samples, 46.9% were positive for PAstV alone whereas 53.1% were co-infected with porcine group A rotavirus (PRVA). A phylogenetic analysis of the partial RNA-dependent RNA polymerase/capsid genes revealed two lineages of PAstV strains detected in this study. PAstV4 was the most dominant genotype (92%), followed by PAstV2 (8%). This study revealed for the first time that PAstV4 and PAstV2 were circulating in Thailand with PAstV4 as the most dominant genotype in pig herds in northern and central parts of Thailand.
猪星状病毒（PAstV）在猪群中广泛分布且高度流行，然而其临床意义仍不明确，因为在腹泻猪和健康猪中均可检测到该病毒。目前尚无关于泰国PAstV的流行情况、临床意义及分子特征的相关信息。本研究采用逆转录聚合酶链反应（RT-PCR），对泰国北部和中部28个猪场采集的488份有腹泻症状和无腹泻症状仔猪的粪便样本进行检测，以调查PAstV的流行情况。PAstV感染的总体流行率为6.5%（32/488），其中腹泻仔猪中PAstV感染率为8.4%（21/251），健康仔猪中为4.6%（11/237）。在32份阳性样本中，仅PAstV阳性的样本占46.9%，而同时感染猪A组轮状病毒（PRVA）的样本占53.1%。对部分RNA依赖性RNA聚合酶/衣壳基因进行系统发育分析，结果显示本研究中检测到的PAstV毒株有两个谱系。PAstV4是最主要的基因型（92%），其次是PAstV2（8%）。本研究首次揭示，PAstV4和PAstV2在泰国流行，且PAstV4是泰国北部和中部猪群中最主要的基因型。
