Li Jin-song, Li Mao-zhong, Zheng Li-shu, Liu Na, Li Dan-di, Duan Zhao-jun
National Institute for Viral Disease Control and Prevention, China CDC, 100 Ying-Xin St., Xuan-Wu District, Beijing, 100052, China.
Arch Virol. 2015 Dec;160(12):3079-84. doi: 10.1007/s00705-015-2569-x. Epub 2015 Sep 4.
Porcine astrovirus are divided into five genotypes. In this study, we identified two porcine astroviruses (AstV-LL-1 and AstV-LL-2) by using sequence-independent single-primer amplification (SISPA) on faecal specimens of healthy domestic piglets younger than 15 days. The detection rate for both was 2.82% (14/497). AstV-LLs were then sequenced and characterised. Phylogenetic analysis revealed that they have the characteristics of porcine astrovirus (PastV) 2 and 5 and have some unique genetic features. Our findings show that the two astroviruses are novel lineages of PAstV2 and 5. The findings may be helpful in evaluating the ecology and evolution of astroviruses in pigs.
猪星状病毒分为五个基因型。在本研究中，我们对15日龄以下健康家猪的粪便样本采用序列非依赖单引物扩增法（SISPA）鉴定出两种猪星状病毒（AstV-LL-1和AstV-LL-2）。二者的检出率均为2.82%（14/497）。随后对AstV-LLs进行了测序和特征分析。系统发育分析表明，它们具有猪星状病毒（PastV）2型和5型的特征，并具有一些独特的遗传特性。我们的研究结果表明，这两种星状病毒是PAstV2和5的新谱系。这些发现可能有助于评估猪星状病毒的生态学和进化情况。
