Aquaculture Research Lab, College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Taian 271018, China.
Aquaculture Research Lab, College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Taian 271018, China.
Ecotoxicol Environ Saf. 2021 Jul 1;217:112266. doi: 10.1016/j.ecoenv.2021.112266. Epub 2021 Apr 27.
Red claw crayfish (Cherax quadricarinatus) is an economically and nutritionally important specie. We aimed to assess the immunostimulatory response to C. quadricarinatus infection with Vibrio parahaemolyticus. After determining the LD50, we infected C. quadricarinatus and examined the differential expression profiles of hepatopancreas transcriptional genes, and observed the temporal changes of hepatopancreas pathological sections and serum immunoenzymatic activities at different time points to reveal the infection mechanism of V. parahaemolyticus and the immune detoxification mechanism of the organism. The results showed that V. parahaemolyticus infection with C. quadricarinatus caused hepatopancreas injury and the immune enzyme activity of the organism changed with time delay. Transcriptome analysis of 47,338 single genes obtained by RNA sequencing and de nove transcriptome assembly identified a total of 3678 differentially expressed genes (P < 0.05) in the expression profiles of susceptible and normal animals for comparative analysis, and 2516 differentially expressed genes (P < 0.05) in the expression profiles of asymptomatic (infection-resistant) and normal animals. GO and KEGG and analyses revealed immune-related pathways under V. parahaemolyticus infection, including Vibrio cholerae infection, phagosome, lysozyme, oxidative phosphorylation, antigen processing and presentation, apoptosis, and Toll-like receptor signaling, as well as significant differences in the expression patterns of related immune genes at different times (P < 0.05). These new experimental results reveal the molecular response of the hepatopancreas to V. parahaemolyticus infection and the corresponding adaptive mechanisms, thus further revealing the pathogenesis due to bacterial infection in the aquatic environment, and providing a reference for further understanding of microbial-host interactions in aquatic systems.
红螯螯虾（Cherax quadricarinatus）是一种具有重要经济和营养价值的物种。我们旨在评估感染副溶血弧菌对红螯螯虾的免疫刺激反应。在确定 LD50 后，我们感染了红螯螯虾，并观察了不同时间点肝胰腺转录基因的差异表达谱以及肝胰腺病理切片和血清免疫酶活性的时变，以揭示副溶血弧菌的感染机制和机体的免疫解毒机制。结果表明，副溶血弧菌感染红螯螯虾引起肝胰腺损伤，机体免疫酶活性随时间延迟而变化。通过 RNA 测序和从头转录组组装获得的 47338 个单基因的转录组分析，在易感动物和正常动物的表达谱中鉴定了总共 3678 个差异表达基因（P < 0.05），在无症状（感染抗性）动物和正常动物的表达谱中鉴定了 2516 个差异表达基因（P < 0.05）。GO 和 KEGG 分析揭示了副溶血弧菌感染下与免疫相关的途径，包括霍乱弧菌感染、吞噬体、溶菌酶、氧化磷酸化、抗原加工和呈递、细胞凋亡和 Toll 样受体信号转导，以及不同时间相关免疫基因表达模式的显著差异（P < 0.05）。这些新的实验结果揭示了肝胰腺对副溶血弧菌感染的分子反应及其相应的适应机制，从而进一步揭示了水生环境中细菌感染引起的发病机制，并为进一步了解水生系统中的微生物-宿主相互作用提供了参考。
