Öner Ibrahim Halil, Querebillo Christine Joy, David Christin, Gernert Ulrich, Walter Carsten, Driess Matthias, Leimkühler Silke, Ly Khoa Hoang, Weidinger Inez M
Professur für Elektrochemie, Technische Universität Dresden, 01062, Dresden, Germany.
Institut für Chemie, Technische Universität Berlin, Strasse des 17. Juni 135, 10623, Berlin, Germany.
Angew Chem Int Ed Engl. 2018 Jun 11;57(24):7225-7229. doi: 10.1002/anie.201802597. Epub 2018 May 16.
We present the fabrication of TiO nanotube electrodes with high biocompatibility and extraordinary spectroscopic properties. Intense surface-enhanced resonance Raman signals of the heme unit of the redox enzyme Cytochrome b were observed upon covalent immobilization of the protein matrix on the TiO surface, revealing overall preserved structural integrity and redox behavior. The enhancement factor could be rationally controlled by varying the electrode annealing temperature, reaching a record maximum value of over 70 at 475 °C. For the first time, such high values are reported for non-directly surface-interacting probes, for which the involvement of charge-transfer processes in signal amplification can be excluded. The origin of the surface enhancement is exclusively attributed to enhanced localized electric fields resulting from the specific optical properties of the nanotubular geometry of the electrode.
我们展示了具有高生物相容性和非凡光谱特性的TiO纳米管电极的制备。在将蛋白质基质共价固定在TiO表面后，观察到氧化还原酶细胞色素b的血红素单元强烈的表面增强共振拉曼信号，揭示了整体保留的结构完整性和氧化还原行为。通过改变电极退火温度可以合理控制增强因子，在475°C时达到超过70的创纪录最大值。首次报道了对于非直接表面相互作用探针有如此高的值，对于这类探针，可以排除电荷转移过程参与信号放大的情况。表面增强的起源完全归因于电极纳米管几何形状的特定光学性质所产生的增强的局部电场。
