Laboratorio de Biotecnología Bacteriana, Instituto de Ciencia y Tecnología de los Alimentos y Nutrición (ICTAN-CSIC), 28040, Madrid, Spain.
National Center for Biotechnology (CNB-CSIC), 28049, Madrid, Spain.
Mol Nutr Food Res. 2018 May;62(9):e1700992. doi: 10.1002/mnfr.201700992. Epub 2018 Apr 30.
This study was undertaken to expand our insights into the mechanisms involved in the tolerance to resveratrol (RSV) that operate at system-level in gut microorganisms and advance knowledge on new RSV-responsive gene circuits.
Whole genome transcriptional profiling was used to characterize the molecular response of Lactobacillus plantarum WCFS1 to RSV. DNA repair mechanisms were induced by RSV and responses were triggered to decrease the load of copper, a metal required for RSV-mediated DNA cleavage, and H S, a genotoxic gas. To counter the effects of RSV, L. plantarum strongly up- or downregulated efflux systems and ABC transporters pointing to transport control of RSV across the membrane as a key mechanism for RSV tolerance. L. plantarum also downregulated tRNAs, induced chaperones, and reprogrammed its transcriptome to tightly control ammonia levels. RSV induced a probiotic effector gene and a likely deoxycholate transporter, two functions that improve the host health status.
Our data identify novel protective mechanisms involved in RSV tolerance operating at system level in a gut microbe. These insights could influence the way RSV is used for a better management of gut microbial ecosystems to obtain associated health benefits.
本研究旨在深入了解肠道微生物中参与白藜芦醇（RSV）耐受的机制，并进一步了解新的 RSV 响应基因回路。
使用全基因组转录谱分析来描述植物乳杆菌 WCFS1 对 RSV 的分子反应。RSV 诱导了 DNA 修复机制，并触发了响应以减少铜的负荷，铜是 RSV 介导的 DNA 切割所需的金属，以及 H₂S，一种遗传毒性气体。为了抵抗 RSV 的影响，植物乳杆菌强烈地上调或下调了外排系统和 ABC 转运蛋白，这表明 RSV 跨膜的转运控制是 RSV 耐受的关键机制。植物乳杆菌还下调了 tRNA，诱导了伴侣蛋白，并重新编程了其转录组以严格控制氨水平。RSV 诱导了一种益生菌效应基因和一种可能的脱氧胆酸盐转运蛋白，这两种功能都能改善宿主的健康状况。
我们的数据确定了在肠道微生物中参与 RSV 耐受的新的保护机制，这些机制可能会影响 RSV 的使用方式，以更好地管理肠道微生物生态系统，从而获得相关的健康益处。
