Key Laboratory of Bio-Pesticide Innovation and Application, Engineering Technology Research Center of Agricultural Pest Biocontrol, South China Agricultural University, Guangzhou, China.
Airport Management College, Guangzhou Civil Aviation College, Guangzhou, China.
Microbiologyopen. 2018 Jun;7(3):e00561. doi: 10.1002/mbo3.561. Epub 2018 Mar 23.
Wolbachia is a group of intracellular bacteria that infect a wide range of arthropods including the Asian citrus psyllid (ACP), Diaphorina citri Kuwayama. This insect is the vector of Candidatus Liberibacter asiaticus (CLas), the causal pathogen of Huanglongbing or citrus greening disease. Here, we investigated the localization pattern and infection dynamics of Wolbachia in different developmental stages of ACP. Results revealed that all developmental stages of ACP including egg, 1st-5th instar nymphs, and adults of both gender were infected with Wolbachia. FISH visualization of an ACP egg showed that Wolbachia moved from the egg stalk of newly laid eggs to a randomly distributed pattern throughout the egg prior to hatching. The infection rate varied between nymphal instars. The titers of Wolbachia in fourth and fifth instar nymphs were significantly higher than those in the first and second instar nymphs. Wolbachia were scattered in all nymphal stages, but with highest intensity in the U-shaped bacteriome located in the abdomen of the nymph. Wolbachia was confined to two symmetrical organizations in the abdomen of newly emerged female and male adults. The potential mechanisms of Wolbachia infection dynamics are discussed.
沃尔巴克氏体是一组感染包括亚洲柑橘木虱（ACP）、柑橘黄龙病菌（HLB）的亚洲韧皮部杆菌在内的多种节肢动物的胞内细菌。本研究旨在探究沃尔巴克氏体在 ACP 不同发育阶段的定位模式和感染动态。结果表明，ACP 的所有发育阶段，包括卵、1-5 龄若虫和雌雄成虫均感染了沃尔巴克氏体。FISH 可视化显示，沃尔巴克氏体从刚产下的卵的卵柄移动到卵内随机分布，然后在孵化前开始移动。感染率在不同龄期之间存在差异。四龄和五龄若虫的沃尔巴克氏体滴度明显高于一龄和二龄若虫。沃尔巴克氏体在所有若虫阶段均有分布，但在位于若虫腹部的 U 形菌体内的分布密度最高。沃尔巴克氏体在新羽化的雌、雄成虫腹部被限制在两个对称的组织中。讨论了沃尔巴克氏体感染动态的潜在机制。
