Laboratory of Oral Surgical Pathology, School of Dentistry, Federal University of Bahia, Av. Araújo Pinho, 62, Canela -, Salvador, Bahia 40110-150, Brazil.
Laboratory of Oral Surgical Pathology, School of Dentistry, Federal University of Bahia, Av. Araújo Pinho, 62, Canela -, Salvador, Bahia 40110-150, Brazil; Center of Biophotonics, School of Dentistry, UFBA, Federal University of Bahia, Av. Araújo Pinho, 62, Canela, Salvador, Bahia 40110-150, Brazil.
J Photochem Photobiol B. 2018 May;182:1-8. doi: 10.1016/j.jphotobiol.2018.03.009. Epub 2018 Mar 16.
The present study aims to assess the influence of Aluminum-Gallium-Indium-Phosphide laser (AlGaInP laser, λ = 660 nm), whether or not in association with the application of a membrane of bacterial cellulose (Nexfill™), during recovery from induced second-degree burns at the dorsum of Wistar rats. (Rattus norvegicus, Wistar). Forty-eight animals have been distributed into four groups: Control (burns remained untreated), Group I (laser-treated), Group II (treated with Nexfill), and Group III (laser + Nexfill™). In addition to a morphological analysis, immunohistochemical analysis has been performed for type I collagen, type III collagen, fibronectin, and laminin. The Fisher's Test was used to assess differences among groups (p < 0,05). A larger amount of collagen type III was observed in Control, Group II and Group III when compared with Group I (p < 0,05). Group I and Group III have shown a greater collagen deposition when compared with Group II (p < 0,05), but the amount of collagen was similar in Group I, Group III, and Control. Group III has shown larger fibronectin amounts in comparison with Group II (p < 0,05). As regards laminin, Group I has shown a predominant discontinuity pattern on the basal lamina in comparison with Control, Group II, and Group III (p < 0,05). It is concluded that in this current study the laser when used alone (Group I) hasn't influenced collagen deposition neither has it acted on fiber pattern (fibril and/or reticular). Moreover, laser application hasn't accelerated the repair of wounds caused by inflicted second-degree burns.
本研究旨在评估铝镓铟磷激光（AlGaInP 激光，λ=660nm）的影响，无论是否联合应用细菌纤维素膜（Nexfill™），以促进 Wistar 大鼠背部诱导的二度烧伤的恢复。（Rattus norvegicus，Wistar）。将 48 只动物分为四组：对照组（烧伤未处理）、I 组（激光治疗）、II 组（用 Nexfill 治疗）和 III 组（激光+Nexfill™）。除了形态学分析外，还进行了 I 型胶原、III 型胶原、纤维连接蛋白和层粘连蛋白的免疫组织化学分析。Fisher 检验用于评估组间差异（p<0.05）。与 I 组相比，对照组、II 组和 III 组观察到更多的 III 型胶原（p<0.05）。与 II 组相比，I 组和 III 组的胶原沉积更多（p<0.05），但 I 组、III 组和对照组的胶原量相似。与 II 组相比，III 组的纤维连接蛋白含量更大（p<0.05）。关于层粘连蛋白，与对照组、II 组和 III 组相比，I 组在基底膜上表现出明显的不连续模式（p<0.05）。本研究得出结论，在当前研究中，单独使用激光（I 组）既没有影响胶原沉积，也没有影响纤维模式（原纤维和/或网状纤维）。此外，激光应用并没有加速因施加二度烧伤引起的伤口的修复。
