College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou 310032, China.
College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou 310032, China.
Comp Biochem Physiol C Toxicol Pharmacol. 2018 Jul;209:1-8. doi: 10.1016/j.cbpc.2018.03.007. Epub 2018 Mar 22.
Lead (Pb) is one of the most prevalent toxic, nonessential heavy metals that has been associated with a wide range of toxic effects in humans and environmental animals. Here, effects of short time exposure to 10 and 30 μg/L Pb on gut microbiota and hepatic metabolism were analyzed in adult male zebrafish. We observed that both 10 and 30 μg/L Pb increased the volume of mucus in the gut. At phylum level, the abundance of α-Proteobacteria decreased significantly and the abundance of Firmicutes increased significantly in the gut when treated with 30 μg/L Pb for 7 days. In addition, the 16S rRNA gene sequencing for V3-V4 region revealed a significant change in the richness and diversity of gut microbiota in 30 μg/L Pb exposed group. A more depth analysis, at the genus level, discovered that 52 gut microbes identified by operational taxonomic unit analysis were changed significantly in 30 μg/L Pb treated group. Based on GC/MS metabolomics analysis, a total of 41 metabolites were significantly altered in 30 μg/L Pb treatment group. These changed metabolites were mainly associated with the pathways of glucose and lipid metabolism, amino acid metabolism, nucleotide metabolism. In addition, we also confirmed that the transcription of some genes related to glycolysis and lipid metabolism, including Gk, Aco, Acc1, Fas, Apo and Dgat, decreased significantly in the liver of zebrafish when exposed to 30 μg/L Pb for 7 days. Our results observed that Pb could cause gut microbiota dysbiosis and hepatic metabolic disorder in zebrafish.
铅（Pb）是一种最普遍的有毒、非必需重金属，它与人类和环境动物的广泛毒性作用有关。在这里，分析了成年雄性斑马鱼短期暴露于 10 和 30μg/L Pb 对肠道微生物群和肝脏代谢的影响。我们观察到，10 和 30μg/L Pb 均增加了肠道内粘液的体积。在门水平上，当用 30μg/L Pb 处理 7 天时，α-变形菌门的丰度显著降低，厚壁菌门的丰度显著增加。此外，V3-V4 区 16S rRNA 基因测序显示，30μg/L Pb 暴露组肠道微生物群的丰富度和多样性发生了显著变化。更深入的分析，在属水平上，发现操作分类单元分析鉴定的 52 种肠道微生物在 30μg/L Pb 处理组中发生了显著变化。基于 GC/MS 代谢组学分析，在 30μg/L Pb 处理组中，共有 41 种代谢物发生了显著改变。这些改变的代谢物主要与葡萄糖和脂质代谢、氨基酸代谢、核苷酸代谢途径有关。此外，我们还证实，在暴露于 30μg/L Pb 7 天后，斑马鱼肝中与糖酵解和脂质代谢相关的一些基因，包括 Gk、Aco、Acc1、Fas、Apo 和 Dgat 的转录水平显著降低。我们的研究结果表明，Pb 可导致斑马鱼肠道微生物群失调和肝脏代谢紊乱。
