State Key Laboratory of Tree Genetics and Breeding & Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, China.
State Key Laboratory of Tree Genetics and Breeding & Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, China.
Gene. 2018 Jun 20;660:120-127. doi: 10.1016/j.gene.2018.03.057. Epub 2018 Mar 22.
Atmospheric carbon dioxide (CO) concentration increases every year. It is critical to understand the elevated CO response molecular mechanisms of plants using genomic techniques. Hippophae rhamnoides L. is a high stress resistance plant species widely distributed in Europe and Asia. However, the molecular mechanism of elevated CO response in H. rhamnoides has been limited. In this study, transcriptomic analysis of two sea buckthorn cultivars under different CO concentrations was performed, based on the next-generation illumina sequencing platform and de novo assembly. We identified 4740 differentially expressed genes in sea buckthorn response to elevated CO concentrations. According to the gene ontology (GO) results, photosystem I, photosynthesis and chloroplast thylakoid membrane were the main enriched terms in 'xiangyang' sea buckthorn. In 'zhongguo' sea buckthorn, photosynthesis was also the main significantly enriched term. However, the number of photosynthesis related differentially expressed genes were different between two sea buckthorn cultivars. Our GO and pathway analyses indicated that the expression levels of the transcription factors WRKY, MYB and NAC were significantly different between the two sea buckthorn cultivars. This study provides a reliable transcriptome sequence resource and is a valuable resource for genetic and genomic researches for plants under high CO concentration in the future.
大气中二氧化碳（CO）浓度逐年增加。利用基因组技术了解植物对升高的 CO 响应的分子机制至关重要。沙棘（Hippophae rhamnoides L.）是一种广泛分布于欧洲和亚洲的高抗逆性植物物种。然而，沙棘对升高的 CO 响应的分子机制还很有限。在这项研究中，我们基于下一代 illumina 测序平台和从头组装，对两个沙棘品种在不同 CO 浓度下的转录组进行了分析。我们在沙棘对升高的 CO 浓度的响应中鉴定出了 4740 个差异表达基因。根据基因本体（GO）结果，在‘向阳’沙棘中，光系统 I、光合作用和叶绿体类囊体膜是主要的富集术语。在‘中国’沙棘中，光合作用也是主要的显著富集术语。然而，两个沙棘品种中与光合作用相关的差异表达基因数量不同。我们的 GO 和通路分析表明，两个沙棘品种中转录因子 WRKY、MYB 和 NAC 的表达水平有显著差异。本研究提供了可靠的转录组序列资源，为未来高 CO 浓度下植物的遗传和基因组研究提供了有价值的资源。
