School of Chemical Engineering, University of New South Wales, Sydney, Australia; Australian Centre for NanoMedicine (ACN), University of New South Wales, Sydney, Australia.
School of Chemical Engineering, University of New South Wales, Sydney, Australia.
J Colloid Interface Sci. 2018 Jul 1;521:242-251. doi: 10.1016/j.jcis.2018.03.006. Epub 2018 Mar 3.
Conjugating nanoparticles with polyethylene glycol (PEG) is a useful strategy to improve the colloidal and biological stability of nanoparticles. However, studies on PEGylation of two-dimensional layered double hydroxide (LDH) nanoparticles are very limited. The present work reported two functionalization approaches to synthesize PEG-conjugated LDH nanoparticles by introducing phosphonic acid terminated PEG before and after LDH aging. The successful PEGylation was confirmed and suggested to be via electrostatic interaction and a ligand exchange process. Different functionalization approaches resulted in different binding types of PEG on/in LDH nanoparticles. The PEG coating maintained the dispersity of LDH nanoparticles in water and saline with the feeding mass ratio of 1:1. Further colloidal stability tests of PEGylated LDHs revealed that the PEGylated LDH dispersity was affected by the feeding mass ratio of PEG/LDH, the molar weight of PEG and anions intercalated in the LDHs. In a test to determine the extent of non-specific protein adsorption, the PEGylation was effective at resisting non-specific bovine serum albumin adsorption on LDH nanoparticles with both functionalization methods investigated. Moreover, PEGylated LDH nanoparticles had no effect on cell viability up to 500 µg/mL, and demonstrated enhanced cellular uptake in a SK-MEL-28 cell culture. The results in this work indicate that conjugating phosphonic acid-terminated PEG on LDH nanoparticles is a promising strategy to improve the colloidal and biological stability of LDHs for biomedical applications.
通过聚乙二醇（PEG）对纳米颗粒进行偶联是提高纳米颗粒胶体和生物稳定性的有效策略。然而，关于二维层状双氢氧化物（LDH）纳米颗粒的 PEG 化研究非常有限。本工作报道了两种功能化方法，通过在 LDH 老化前后引入末端为磷酸的 PEG 来合成 PEG 接枝的 LDH 纳米颗粒。成功的 PEG 化得到了证实，并被认为是通过静电相互作用和配体交换过程实现的。不同的功能化方法导致 PEG 在 LDH 纳米颗粒上的结合类型不同。PEG 涂层保持了 LDH 纳米颗粒在水和盐水中的分散性，PEG/LDH 的进料质量比为 1:1。进一步对 PEG 化 LDH 的胶体稳定性测试表明，PEG 化 LDH 的分散性受到 PEG/LDH 的进料质量比、PEG 的摩尔质量和 LDH 中插层阴离子的影响。在确定非特异性蛋白质吸附程度的测试中，两种研究的功能化方法都证明 PEG 化可有效抵抗 LDH 纳米颗粒上非特异性牛血清白蛋白的吸附。此外，PEG 化 LDH 纳米颗粒在 500µg/mL 以下对细胞活力没有影响，并在 SK-MEL-28 细胞培养中显示出增强的细胞摄取。本工作的结果表明，在 LDH 纳米颗粒上接枝末端为磷酸的 PEG 是提高 LDH 胶体和生物稳定性用于生物医学应用的一种有前途的策略。
