Methylglyoxal Alone or Combined with Light-Emitting Diodes/Complex Electromagnetic Fields Represent an Effective Response to Microbial Chronic Wound Infections.

antimicrobial resistance represents a critical issue leading to delayed wound healing; hence, it is necessary to develop novel strategies to address this phenomenon. this study aimed to explore the antimicrobial/anti-virulence action of Methylglyoxal-MGO alone or combined with novel technologies such as Light-Emitting Diodes-LED and Complex Magnetic Fields-CMFs against resistant clinical strains isolated from chronic wounds. characterized planktonic , , and isolates were used. Antimicrobial activity was evaluated by measuring optical density, Colony Forming Units-CFU, and synergy between MGO/LED or CMFs. Cellular membrane permeability by propidium iodide fluorescence and fluidity by Laurdan generalized polarization measurements were performed. motility was tested using the soft agar method. A docking study was performed to evaluate the possible interaction between MGO and urease in . single/combined treatments showed significant antimicrobial activity. Major CFU reduction was detected after CMFs/MGO+CMFs application on . Treatments exhibited significant changes in membrane permeability and fluidity. The treatments decreased motility with a major reduction after LED application. Docking analysis showed that MGO could bind with urease leading to defective folding and functional alterations. the results suggest that these treatments could represent promising and green therapeutic solutions against resistant isolates from chronic wounds.