Lopez-Monroy Beatriz, Gutierrez-Rodriguez Selene M, Villanueva-Segura Olga Karina, Ponce-Garcia Gustavo, Morales-Forcada Franco, Alvarez Leslie C, Flores Adriana E
Universidad Autonoma de Nuevo Leon, Facultad de Ciencias Biologicas, Departamento de Zoologia de Invertebrados, San Nicolas de los Garza, Mexico.
Universidad de los Andes, Nucleo Universitario Rafael Rangel, estado Trujillo, Venezuela.
Pest Manag Sci. 2018 Mar 25. doi: 10.1002/ps.4916.
The control of Aedes aegypti (L.), the main urban vector that causes arboviral diseases such as dengue, Chikungunya and Zika, has proved to be a challenge because of a rapid increase in insecticide resistance. Therefore, adequate monitoring of insecticide resistance is an essential element in the control of Ae. aegypti and the diseases it transmits. We estimated the frequency and intensity (Resistance Frequency Rapid Diagnostic Test [F-RDT] and Resistance Intensity Rapid Diagnostic Test [I-RDT]) of pyrethroid resistance in populations of Ae. aegypti from Mexico using the bottle bioassay and results were related to the frequencies of knockdown resistance (kdr) mutations V1016I and F1534C.
All populations under study were resistant to the pyrethroids: bifenthrin (99%), d-(cis-trans)-phenothrin (6.3% cis, 91.7% trans) and permethrin (99.5%) according to F-RDT, and showed moderate to high-intensity resistance at 10× the diagnostic dose (DD) in I-RDT. Frequencies of the kdr mutation V1016I in Ae. aegypti populations were correlated with moderate permethrin resistance at 10× DD, whereas F1534C mutation frequencies were correlated with high bifenthrin resistance at 5× DD. Both I1016 and C1535 were highly correlated with high-intensity phenothrin resistance at 1× to 10× DD.
This study showed that high frequencies of kdr mutations V1016I and F1534C are reflected in the results of F-RDT and I-RDT tests. Bioassays in conjunction with the characterization of genetic resistance mechanisms are indispensable in the strategic and rational management of resistance in mosquitoes. © 2018 Society of Chemical Industry.
埃及伊蚊是导致登革热、基孔肯雅热和寨卡等虫媒病毒疾病的主要城市病媒，由于杀虫剂抗性迅速增加，对其进行控制已被证明是一项挑战。因此，对杀虫剂抗性进行充分监测是控制埃及伊蚊及其传播疾病的关键要素。我们使用瓶式生物测定法估计了墨西哥埃及伊蚊种群中拟除虫菊酯抗性的频率和强度（抗性频率快速诊断测试 [F-RDT] 和抗性强度快速诊断测试 [I-RDT]），并将结果与击倒抗性（kdr）突变V1016I和F1534C的频率相关联。
根据F-RDT，所有研究种群均对拟除虫菊酯产生抗性：联苯菊酯（99%）、d-（顺式-反式）苯醚菊酯（顺式6.3%，反式91.7%）和氯菊酯（99.5%），并且在I-RDT中于诊断剂量（DD）的10倍时表现出中度至高抗性强度。埃及伊蚊种群中kdr突变V1016I的频率与氯菊酯在10×DD时的中度抗性相关，而F1534C突变频率与联苯菊酯在5×DD时的高抗性相关。I1016和C1535均与苯醚菊酯在1×至10×DD时的高强度抗性高度相关。
本研究表明，kdr突变V1016I和F1534C的高频率反映在F-RDT和I-RDT测试结果中。生物测定结合遗传抗性机制的表征在蚊虫抗性的战略和合理管理中必不可少。© 2018化学工业协会。
