Tabari Zahra Alizadeh, Keshani Forooz, Sharbatdaran Majid, Banishahabadi Amirhossein, Nejatifard Marziyeh, Ghorbani Hossein
Department of Periodontics, Dental School, Qazvin University of Medical Sciences, Qazvin, Iran.
Department of Oral and Maxillofacial Pathology, Dental Research Center, School of Dentistry, Isfahan University of Medical Science, Isfahan, Iran.
Dent Res J (Isfahan). 2018 Mar-Apr;15(2):104-110.
Visfatin, also known as nicotinamide phosphoribosyltransferase, has been suggested as a pro-inflammatory and immunomodulating marker for periodontitis. The aim of this study was an immunohistochemical analysis of visfatin in gingival tissues of patients with chronic periodontitis and aggressive periodontitis.
In this cross-sectional study based on clinical evaluation and inclusion and exclusion criteria. Twenty patients with generalized chronic periodontitis, 13 patients with generalized aggressive periodontitis, and 20 periodontally healthy individuals enrolled. Gingival tissue samples were obtained during periodontal flap surgery and crown lengthening surgery in periodontal patients and healthy group, respectively. Tissue samples were transferred to a pathology laboratory to determine the degree of inflammatory infiltration by hematoxylin and eosin staining and the level of visfatin expression by immunohistochemistry. The data were analyzed using SPSS version 20 statistical software and paired -test, Mann-Whitney test, and Spearman rank correlation coefficient. < 0.05 was considered statistically significant.
Inflammation grading and visfatin expression were significantly higher in periodontally diseased gingiva compared to the control group ( < 0.05). However, no significant difference was found between two diseased groups. The relationship between inflammation grading and visfatin expression in aggressive periodontitis group was statistically significant, positive and relatively strong ( = 0.025, = 0.617). However, no significant relationship has been found between visfatin expression and inflammation grading in the chronic periodontitits and control groups ( > 0.05).
Visfatin expression was increased in gingival tissues of chronic periodontitis and aggressive periodontitis patients. Hence, visfatin may have a role in the etiopathogenesis of chronic periodontitis and aggressive periodontitis.
内脂素，也被称为烟酰胺磷酸核糖转移酶，已被认为是牙周炎的一种促炎和免疫调节标志物。本研究的目的是对慢性牙周炎和侵袭性牙周炎患者牙龈组织中的内脂素进行免疫组织化学分析。
在这项基于临床评估以及纳入和排除标准的横断面研究中。纳入了20例广泛性慢性牙周炎患者、13例广泛性侵袭性牙周炎患者和20例牙周健康个体。分别在牙周炎患者和健康组进行牙周翻瓣手术和牙冠延长手术时获取牙龈组织样本。将组织样本送至病理实验室，通过苏木精和伊红染色确定炎症浸润程度，并通过免疫组织化学确定内脂素表达水平。使用SPSS 20版统计软件进行数据分析，并采用配对检验、曼-惠特尼检验和斯皮尔曼等级相关系数。P<0.05被认为具有统计学意义。
与对照组相比，牙周病损牙龈中的炎症分级和内脂素表达显著更高（P<0.05）。然而，两个病损组之间未发现显著差异。侵袭性牙周炎组中炎症分级与内脂素表达之间的关系具有统计学意义，呈正相关且相对较强（P = 0.025，r = 0.617）。然而，在慢性牙周炎组和对照组中，内脂素表达与炎症分级之间未发现显著关系（P>0.05）。
慢性牙周炎和侵袭性牙周炎患者的牙龈组织中内脂素表达增加。因此，内脂素可能在慢性牙周炎和侵袭性牙周炎的发病机制中起作用。
