Kong Fanhong, Zhang Wenli, Qiao Lin, Li Qi, Li Haowen, Cao Jingli, He Wenyan, Dong Chengya, He Yanjiao, He Lu, Liu Li, Fu Weilun, Liu Lijun, Li Zirui, Wang Yajie
Beijing Tiantan Hospital, Capital Medical University, Beijing, China.
Clinical Medical Research Laboratory, Beijing Tiantan Hospital, Capital Medical University, Beijing, China.
PeerJ. 2018 Mar 13;6:e4450. doi: 10.7717/peerj.4450. eCollection 2018.
We established a glioma biobank at Beijing Tiantan Hospital in November, 2010. Specialized residents have been trained to collect, store and manage the biobank in accordance with standard operating procedures.
One hundred samples were selected to evaluate the quality of glioma samples stored in the liquid nitrogen tank during different periods (from 2011 to 2015) by morphological examination, RNA integrity determination, DNA integrity determination and housekeeping gene expression determination.
The majority of samples (95%) had high RNA quality for further analysis with RIN ≥6. Quality of DNA of all samples were stable without significant degradation.
Storage conditions of our biobank are suitable for long-term (at least five years) sample preservation with high molecular quality.
2010年11月，我们在北京天坛医院建立了一个胶质瘤生物样本库。已对专业住院医师进行培训，使其按照标准操作程序收集、储存和管理该生物样本库。
选取100个样本，通过形态学检查、RNA完整性测定、DNA完整性测定和管家基因表达测定，评估在不同时期（2011年至2015年）储存在液氮罐中的胶质瘤样本的质量。
大多数样本（95%）具有较高的RNA质量，RIN≥6，可用于进一步分析。所有样本的DNA质量稳定，无明显降解。
我们生物样本库的储存条件适合长期（至少五年）保存高分子质量的样本。
