Laboratory of Clinical Biochemistry, Department of Clinical and Toxicological Analysis, Federal University of Santa Maria, Santa Maria, RS, Brazil.
Laboratory of Clinical Biochemistry, Department of Clinical and Toxicological Analysis, Federal University of Santa Maria, Santa Maria, RS, Brazil; University Hospital, Federal University of Santa Maria, Santa Maria, RS, Brazil.
Clin Chim Acta. 2018 Jul;482:46-49. doi: 10.1016/j.cca.2018.03.026. Epub 2018 Mar 23.
Gamma-glutamyltransferase (GGT) is present mainly in proximal renal tubule, and urinary GGT is an indicator of tubular damage since it may show renal changes before they are identified by using conventional measurements. Therefore, it is of interest to establish the reference limits of urinary GGT for a healthy population, as well as to investigate the stability of GGT in urine samples stored at 4 °C and -20 °C.
GGT was assessed in urine samples from 127 healthy patients by use of a reference method based on the 5-Amino-2-Nitrobenzoate formation. Stability of GGT was evaluated in 10 urine samples stored at temperatures of 4 °C and -20 °C for a period up to 4 weeks.
Urinary GGT values for healthy volunteers were 14 U/g creatinine for the lower reference limit and 79 U/g creatinine for the upper reference limit. Urinary GGT values were approximately 56% lower in samples stored at -20 °C than fresh samples, while samples stored at 4 °C presented a decrease of 11% in GGT values compared to fresh samples.
Reference limits for urinary GGT in healthy subjects were 14 to 79 U/g creatinine, and it is recommended to measure urinary GGT in fresh specimens.
γ-谷氨酰转移酶（GGT）主要存在于近端肾小管中，尿 GGT 是肾小管损伤的指标，因为它可能在常规测量之前显示肾脏变化。因此，确定健康人群尿 GGT 的参考范围以及研究 4°C 和-20°C 储存的尿样中 GGT 的稳定性很有意义。
采用基于 5-氨基-2-硝基苯甲酸形成的参考方法评估了 127 例健康患者的尿 GGT。在长达 4 周的时间内，将 10 份尿液样本分别储存在 4°C 和-20°C 下，评估 GGT 的稳定性。
健康志愿者的尿 GGT 值下限为 14 U/g 肌酐，上限为 79 U/g 肌酐。与新鲜样本相比，储存在-20°C 的样本中尿 GGT 值降低了约 56%，而储存在 4°C 的样本中 GGT 值降低了 11%。
健康受试者尿 GGT 的参考范围为 14 至 79 U/g 肌酐，建议在新鲜标本中测量尿 GGT。
