储存时间和温度对尿酶的影响。

Effects of storage time and temperature on urinary enzymes.

作者信息

Matteucci E, Gregori G, Pellegrini L, Navalesi R, Giampietro O

机构信息

Cattedra di Malattie del Metabolismo, Istituto di Clinica Medica II, Università degli Studi di Pisa, Italy.

出版信息

Clin Chem. 1991 Aug;37(8):1436-41.

PMID:1678320

Abstract

We measured three renal tubular brush-border enzymes (lactate dehydrogenase, LDH, EC 1.1.1.27; gamma-glutamyltransferase, GGT, EC 2.3.2.2; and alkaline phosphatase, AP, EC 3.1.3.1) in morning urine samples from 48 healthy subjects to check whether different storage times and temperatures could modify enzyme concentrations. Short-term (24 h) storage time at room temperature or 4 degree C does not affect urinary enzyme activity. A few days of freezing, at -20 or -70 degrees C, dramatically lowers LDH and AP values; GGT is partially preserved only at -70 degrees C, if the sample has been previously centrifuged. Urinary enzymes investigated in this study are extremely labile at low temperatures.

摘要

我们检测了48名健康受试者晨尿样本中的三种肾小管刷状缘酶（乳酸脱氢酶，LDH，EC 1.1.1.27；γ-谷氨酰转移酶，GGT，EC 2.3.2.2；碱性磷酸酶，AP，EC 3.1.3.1），以检查不同的储存时间和温度是否会改变酶浓度。室温或4℃下短期（24小时）储存时间不影响尿酶活性。在-20℃或-70℃下冷冻几天会显著降低LDH和AP值；只有在样本预先离心的情况下，GGT仅在-70℃下部分保留。本研究中检测的尿酶在低温下极不稳定。