Centre for Nanoscience and Molecular Medicine, Amrita Institute of Medical Sciences and Research Centre, Amrita Vishwa Vidyapeetham, Kochi 682041, India.
Centre for Nanoscience and Molecular Medicine, Amrita Institute of Medical Sciences and Research Centre, Amrita Vishwa Vidyapeetham, Kochi 682041, India.
Int J Biol Macromol. 2018 Jun;112:737-744. doi: 10.1016/j.ijbiomac.2018.02.031. Epub 2018 Feb 7.
Micropatterning techniques involve soft lithography, which is laborious, expensive and restricted to a narrow spectrum of biomaterials. In this work we report, first time employment of patterned wax moulds for generation of micropatterned alginate-gelatin and κ-carrageenan (κ-CRG) hydrogel systems by a novel, simple and cost effective method. We generated and characterized uniform and reproducible micropatterned hydrogels of varying sizes and shapes such as square projections, square grooves, and circular grids and crisscrossed hillocks. The rheological analysis showed that κ-carrageenan hydrogels had higher gel strength when compared to alginate-gelatin hydrogels. Human Mesenchymal stem cells (hMSCs) and Human Induced Pluripotent Stem Cells (hiPSCs) were found to be cytocompatible with these hydrogels. This micropatterned hydrogel system may have potential application in tissue engineering and also in understanding the basic biology behind the stem cell/iPSC fate.
微图案化技术涉及软光刻，该技术既费力又昂贵，并且仅限于狭窄的生物材料谱。在这项工作中，我们首次报告了使用图案化的蜡模通过新颖、简单且具有成本效益的方法来生成微图案化的藻酸盐-明胶和 κ-卡拉胶（κ-CRG）水凝胶系统。我们生成并表征了具有不同尺寸和形状的均匀且可重复的微图案化水凝胶，例如方形突出物、方形凹槽和圆形网格以及交错的丘陵。流变分析表明，与藻酸盐-明胶水凝胶相比，κ-卡拉胶水凝胶具有更高的凝胶强度。发现人骨髓间充质干细胞（hMSCs）和人诱导多能干细胞（hiPSCs）与这些水凝胶具有细胞相容性。这种微图案化水凝胶系统在组织工程中以及在理解干细胞/iPSC 命运背后的基本生物学方面可能具有潜在的应用。
