吉林省和辽宁省家养兔（Oryctolagus cuniculus）中十二指肠贾第虫的流行情况及危险因素。
Prevalence and risk factors of Giardia duodenalis in domestic rabbbits (Oryctolagus cuniculus) in Jilin and Liaoning province, northeastern China.
机构信息
College of Animal Science and Technology, Chanchun Sci-Tech University, Shuangyang, Jilin Province 130600, PR China.
College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi Province, 712100, PR China; College of Animal Science and Technology, Jilin Agricultural University, Changchun, Jilin Province 130118, PR China.
出版信息
J Infect Public Health. 2018 Sep-Oct;11(5):723-726. doi: 10.1016/j.jiph.2018.02.008. Epub 2018 Mar 23.
BACKGROUND
Giardia duodenalis is one of the most important intestinal parasites that can infect virtually all animals, including rabbits and humans. However, there is little information regarding the prevalence and genotypes of G. duodenalis in domestic rabbits in China.
METHODS
A total of 426 rabbit fecal samples (136 from Shenyang City, 174 from Changchun City, and 116 from Jilin City) were examined by Lugol's iodine staining with microscopy analysis, and the positive samples were genotyped at the triosephosphate isomerase (tpi) and the beta giardin (bg) gene loci using nested PCR.
RESULTS
Forty-two (9.86%) out of 426 rabbit fecal samples were G. duodenalis-positive under microscopy analysis, and the highest G. duodenalis infection rate was 23.08% on farm 6. The prevalence of G. duodenalis in rabbits from different cities ranged from 1.47% to 14.37%. Among different age groups, G. duodenalis prevalence in rabbits ranged from 5.41% to 12.58%. The prevalence of G. duodenalis in outdoor rabbits and indoor rabbits was 14.29% and 6.77%, respectively. In the present study, region and farming mode were highly correlated with G. duodenalis infection in rabbits. All 42 G. duodenalis isolates were successfully amplified and sequenced at the tpi and bg loci, and only G. duodenalis assemblage B were identified.
CONCLUSION
This study not only further confirmed the dominance of G. duodenalis assemblage B in rabbits, but also further improved the foundation data concerning the distribution of G. duodenalis assemblages in China.
背景
十二指肠贾第鞭毛虫是一种可以感染几乎所有动物的重要肠道寄生虫，包括兔子和人类。然而，关于中国家兔中贾第鞭毛虫的流行情况和基因型，信息很少。
方法
用卢戈氏碘液染色显微镜分析法检查了 426 份兔粪便样本（沈阳市 136 份，长春市 174 份，吉林市 116 份），并通过巢式 PCR 在三磷酸甘油异构酶（tpi）和β微管蛋白（bg）基因座上对阳性样本进行基因分型。
结果
在显微镜分析下，426 份兔粪便样本中有 42 份（9.86%）为十二指肠贾第鞭毛虫阳性，其中 6 号农场的感染率最高，为 23.08%。不同城市的兔感染率在 1.47%至 14.37%之间。不同年龄组的兔感染率在 5.41%至 12.58%之间。户外兔和室内兔的感染率分别为 14.29%和 6.77%。本研究中，地区和养殖方式与兔感染贾第鞭毛虫高度相关。在 tpi 和 bg 基因座上成功扩增和测序了 42 株贾第鞭毛虫分离株，仅鉴定出了贾第鞭毛虫 B 型。
结论
本研究不仅进一步证实了贾第鞭毛虫 B 型在家兔中占主导地位，还进一步提高了中国贾第鞭毛虫属分布的基础数据。
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