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全球赖氨酸乙酰化和 2-羟基异丁酰化谱分析揭示了贾第虫代谢转换机制。

Global Lysine Acetylation and 2-Hydroxyisobutyrylation Profiling Reveals the Metabolism Conversion Mechanism in Giardia lamblia.

机构信息

Academy of Basic Medicine, Jilin Medical University, Jilin, China.

Academy of Basic Medicine, Jilin Medical University, Jilin, China.

出版信息

Mol Cell Proteomics. 2021;20:100043. doi: 10.1074/mcp.RA120.002353. Epub 2021 Jan 7.

DOI:10.1074/mcp.RA120.002353
PMID:33376196
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8724866/
Abstract

Giardia lamblia (G. lamblia) is the cause of giardiasis, a common infection that affects the general population of the world. Despite the constant possibility of damage because of their own metabolism, G. lamblia has survived and evolved to adapt to various environments. However, research on energy-metabolism conversion in G. lamblia is limited. This study aimed to reveal the dynamic metabolism conversion mechanism in G. lamblia under sugar starvation by detecting global lysine acetylation (Kac) and 2-hydroxyisobutyrylation (Khib) sites combined with quantitative proteome analyses. A total of 2999 acetylation sites on 956 proteins and 8877 2-hydroxyisobutyryl sites on 1546 proteins were quantified under sugar starvation. Integrated Kac and Khib data revealed that modified proteins were associated with arginine biosynthesis, glycolysis/gluconeogenesis, and alanine, aspartate, and glutamate metabolisms. These findings suggest that Kac and Khib were ubiquitous and provide deep insight into the metabolism conversion mechanism in G. lamblia under sugar starvation. Overall, these results can help delineate the biology of G. lamblia infections and reveal the evolutionary rule from prokaryote to eukaryote.

摘要

蓝氏贾第鞭毛虫（G. lamblia）是贾第虫病的病原体，一种常见的感染，影响着世界上的普通人群。尽管由于自身代谢不断有损伤的可能性，但 G. lamblia 已经存活并进化以适应各种环境。然而，对 G. lamblia 能量代谢转换的研究是有限的。本研究旨在通过检测全球赖氨酸乙酰化（Kac）和 2-羟基异丁酸酰化（Khib）位点并结合定量蛋白质组分析，揭示糖饥饿下 G. lamblia 中的动态代谢转换机制。在糖饥饿下，共定量了 2999 个位于 956 种蛋白质上的乙酰化位点和 8877 个位于 1546 种蛋白质上的 2-羟基异丁酸酰化位点。综合 Kac 和 Khib 数据表明，修饰蛋白与精氨酸生物合成、糖酵解/糖异生以及丙氨酸、天冬氨酸和谷氨酸代谢有关。这些发现表明 Kac 和 Khib 是普遍存在的，并为深入了解糖饥饿下 G. lamblia 的代谢转换机制提供了线索。总的来说，这些结果可以帮助描绘 G. lamblia 感染的生物学特性，并揭示从原核生物到真核生物的进化规律。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7290/8724866/1927e0fc4d0e/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7290/8724866/ee2c43b7d8d2/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7290/8724866/7eaefebdcc52/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7290/8724866/971df2e80cce/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7290/8724866/46b98bd64134/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7290/8724866/d2eb72a8ddb7/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7290/8724866/d1284b988c6b/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7290/8724866/15199c011d1b/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7290/8724866/1927e0fc4d0e/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7290/8724866/ee2c43b7d8d2/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7290/8724866/7eaefebdcc52/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7290/8724866/971df2e80cce/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7290/8724866/46b98bd64134/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7290/8724866/d2eb72a8ddb7/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7290/8724866/d1284b988c6b/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7290/8724866/15199c011d1b/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7290/8724866/1927e0fc4d0e/gr7.jpg

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