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山东省东部地区兔源 的流行情况和基因型分布。

Prevalence and Genotype Distribution of in Rabbits in Shandong Province, Eastern China.

机构信息

College of Veterinary Medicine, Gansu Agricultural University, Lanzhou, Gansu 730070, China.

State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu 730046, China.

出版信息

Biomed Res Int. 2020 Feb 17;2020:4714735. doi: 10.1155/2020/4714735. eCollection 2020.

Abstract

is a zoonotic enteric parasite that can infect humans and a number of animal species including rabbits with a worldwide distribution. Infection with can cause serious public health problems and significant economic losses to animal husbandry. So accurate understanding of the prevalence and genotype distribution of in rabbits is necessary. In the present study, a total of 616 fecal samples were collected from rabbits in Shandong province, eastern China, and examined in prevalence and genotypes by nested PCR amplification of -giardin (bg), glutamate dehydrogenase (gdh), and triosephosphate isomerase (tpi) gene loci of . Sixty-nine (11.2%) of the examined rabbit fecal samples were positive. Of them, the prevalence of is 8.4% (41/490) in Rizhao city and 22.2% (28/126) in Weihai city. Breeds, region, and feeding modes were highly correlated with infection in rabbits. Moreover, three genotypes (assemblages A, B, and E) were identified in rabbits at three gene loci, and the assemblage E was the dominant genotype, while the assemblage A was reported in rabbits in China for the first time. It is noticeable that two rabbits were found to be infected with two different assemblages (assemblages A and E, assemblages B and E, respectively). These findings enrich the genotype distribution of in rabbits and provide baseline data for preventing and controlling infection in rabbits in eastern China.

摘要

是一种人畜共患的肠道寄生虫,可感染人类和包括兔子在内的多种动物,分布于世界各地。感染可导致严重的公共卫生问题,并给畜牧业造成重大经济损失。因此,准确了解兔子中感染的流行情况和基因型分布是必要的。本研究共采集中国东部山东省 616 份兔粪便样本,通过巢式 PCR 扩增 -giardin (bg)、谷氨酸脱氢酶 (gdh) 和磷酸丙糖异构酶 (tpi) 基因座,检测的 69 份(11.2%)兔粪便样本呈阳性。其中,日照市的感染率为 8.4%(41/490),威海市的感染率为 22.2%(28/126)。品种、地区和饲养方式与兔子的感染高度相关。此外,在兔子的三个基因座上鉴定出了三种基因型(A、B 和 E 组),其中 E 组是优势基因型,而 A 组是中国首次在兔子中报道。值得注意的是,有两只兔子被发现感染了两种不同的(A 组和 E 组、B 组和 E 组)。这些发现丰富了兔子中感染的基因型分布,并为中国东部地区预防和控制兔子感染提供了基线数据。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/54e6/7048920/db4667863b90/BMRI2020-4714735.001.jpg

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