Nucleus of Tropical Medicine, Federal University of Pará, Generalíssimo Deodoro Av., District Umarizal, Belém, PA 66.055-240, Brazil.
Evandro Chagas Institute, BR-316 KM 7 Highway, Ananindeua, PA 67.030-000, Brazil.
Genomics. 2019 Jul;111(4):607-611. doi: 10.1016/j.ygeno.2018.03.016. Epub 2018 Mar 24.
Mosquitoes as Sabethes chloropterus, Sabethes glaucodaemon, Sabethes belisarioi are species of medical and epidemiological importance for arboviruses transmission such as yellow fever and St. Louis encephalitis. Despite this, no information about these three species mitochondrial DNA has been found in literature. Our study presents a mitochondrial genome description, including identity, SNPs, mutation rate, and phylogeny analysis using COX1, COX2, NADH4, NADH5, CYOB genes. The Sa. chloropterus, Sa. glaucodaemon and Sa. belisaroi mitochondrial genome sizes 15.609 bp, 15.620 bp, 15.907 bp, respectively, with 37 functional genes, presenting about 4.982 single nucleotide polymorphisms and 13.291 identical sites between them, besides all genes with dN/dS < 1 ratio, and also a greater approximation between Sa. glaucodaemon and Sa. chloropterus than with Sa. belisarioi. Due to the importance of mitochondrial DNA for population structure studies, evolution, and others, we expect that this data can contribute to other studies related to these mosquitoes and their viruses.
作为医学和流行病学上具有重要意义的传播媒介,塞蚊亚属的 Sabethes chloropterus、Sabethes glaucodaemon 和 Sabethes belisarioi 能够传播黄热病和圣路易斯脑炎等虫媒病毒。尽管如此,目前文献中尚未发现这三个物种的线粒体 DNA 信息。本研究使用 COX1、COX2、NADH4、NADH5 和 CYOB 基因,对 S. chloropterus、S. glaucodaemon 和 S. belisarioi 的线粒体基因组进行了描述,包括其身份、单核苷酸多态性、突变率和系统发育分析。Sa. chloropterus、Sa. glaucodaemon 和 Sa. belisaroi 的线粒体基因组大小分别为 15.609bp、15.620bp 和 15.907bp,包含 37 个功能基因,它们之间有大约 4982 个单核苷酸多态性和 13291 个相同位点,此外所有基因的 dN/dS<1,且 Sa. glaucodaemon 和 Sa. chloropterus 之间的相似度高于 Sa. belisarioi。鉴于线粒体 DNA 对于种群结构研究、进化等方面的重要性,我们希望这些数据能够为与这些蚊子及其病毒相关的其他研究提供参考。