玉米赤霉烯酮在体外经母猪肠黏膜的代谢

Metabolism of zearalenone by sow intestinal mucosa in vitro.

作者信息

Olsen M, Pettersson H, Sandholm K, Visconti A, Kiessling K H

机构信息

Department of Animal Nutrition and Management, Swedish University of Agricultural Sciences, Uppsala.

出版信息

Food Chem Toxicol. 1987 Sep;25(9):681-3. doi: 10.1016/0278-6915(87)90101-3.

DOI:10.1016/0278-6915(87)90101-3

PMID:2958396

Abstract

Homogenized intestinal mucosa samples from sows were incubated with zearalenone in the presence of NADPH or UDPGA. In addition, UDPglucuronosyltransferase activity in the microsomal fraction of mucosa was determined using 1-naphthol as substrate. In the presence of NADPH, zearalenone was reduced to both alpha- and beta-zearalenol (0.37 +/- 0.18 and 0.29 +/- 0.11 nmol/mg protein/hr in the duodenum and jejunum, respectively). The beta-isomer was the predominant metabolite. Glucuronide conjugation of zearalenone was very high compared with the level of reduction occurring (11.3 +/- 6.1 and 9.4 +/- 5.8 nmol conjugated/mg protein/hr in the duodenum and jejunum, respectively). There was no correlation between the rates of glucuronide conjugation of zearalenone and 1-naphthol, indicating that they depend upon two different isoenzymes of UDPglucuronosyltransferase.

摘要

将母猪的匀浆肠黏膜样本与玉米赤霉烯酮在NADPH或UDPGA存在的情况下进行孵育。此外，以1-萘酚为底物测定黏膜微粒体部分中的UDP葡萄糖醛酸基转移酶活性。在NADPH存在的情况下，玉米赤霉烯酮被还原为α-玉米赤霉醇和β-玉米赤霉醇（十二指肠和空肠中分别为0.37±0.18和0.29±0.11 nmol/毫克蛋白质/小时）。β-异构体是主要代谢产物。与还原水平相比，玉米赤霉烯酮的葡萄糖醛酸结合非常高（十二指肠和空肠中分别为11.3±6.1和9.4±5.8 nmol结合物/毫克蛋白质/小时）。玉米赤霉烯酮和1-萘酚的葡萄糖醛酸结合速率之间没有相关性，表明它们依赖于UDP葡萄糖醛酸基转移酶的两种不同同工酶。