Georgopoulou Anthie, Kaliva Maria, Vamvakaki Maria, Chatzinikolaidou Maria
Department of Materials Science and Technology, University of Crete, Voutes Campus, 70013 Heraklion, Greece.
Institute of Electronic Structure and Laser, Foundation for Research and Technology-Hellas, Nikolaou Plastira 100, 70013 Heraklion, Greece.
Materials (Basel). 2018 Mar 26;11(4):490. doi: 10.3390/ma11040490.
A chitosan--polycaprolactone (CS--PCL) copolymer synthesized via a multi-step process was evaluated as a potential biomaterial for the adhesion and growth of MC3T3-E1 pre-osteoblastic cells. A strong adhesion of the MC3T3-E1 cells with a characteristic spindle-shaped morphology was observed from the first days of cell culture onto the copolymer surfaces. The viability and proliferation of the cells on the CS--PCL surfaces, after 3 and 7 days in culture, were significantly higher compared to the cells cultured on the tissue culture treated polystyrene (TCPS) control. The osteogenic potential of the pre-osteoblastic cells cultured on CS--PCL surfaces was evaluated by determining various osteogenic differentiation markers and was compared to the TCPS control surface. Specifically, alkaline phosphatase activity levels show significantly higher values at both time points compared to TCPS, while secreted collagen into the extracellular matrix was found to be higher on day 7. Calcium biomineralization deposited into the matrix is significantly higher for the CS--PCL copolymer after 14 days in culture, while the levels of intracellular osteopontin were significantly higher on the CS--PCL surfaces compared to TCPS. The enhanced osteogenic response of the MC3T3-E1 pre-osteoblasts cultured on CS--PCL reveals that the copolymer underpins the cell functions towards bone tissue formation and is thus an attractive candidate for use in bone tissue engineering.
通过多步工艺合成的壳聚糖-聚己内酯（CS-PCL）共聚物被评估为用于MC3T3-E1前成骨细胞黏附和生长的潜在生物材料。从细胞培养的第一天起，就观察到MC3T3-E1细胞以特征性的纺锤形形态强烈黏附在共聚物表面。在培养3天和7天后，与在组织培养处理的聚苯乙烯（TCPS）对照上培养的细胞相比，CS-PCL表面上细胞的活力和增殖明显更高。通过测定各种成骨分化标志物来评估在CS-PCL表面上培养的前成骨细胞的成骨潜力，并与TCPS对照表面进行比较。具体而言，与TCPS相比，在两个时间点碱性磷酸酶活性水平均显示出明显更高的值，而在第7天发现分泌到细胞外基质中的胶原蛋白更高。培养14天后，CS-PCL共聚物沉积到基质中的钙生物矿化明显更高，而与TCPS相比，CS-PCL表面上细胞内骨桥蛋白的水平明显更高。在CS-PCL上培养的MC3T3-E1前成骨细胞的成骨反应增强，表明该共聚物支持细胞对骨组织形成的功能，因此是骨组织工程中极具吸引力的候选材料。
