Kaliva Maria, Georgopoulou Anthie, Dragatogiannis Dimitrios A, Charitidis Costas A, Chatzinikolaidou Maria, Vamvakaki Maria
Institute of Electronic Structure and Laser, Foundation for Research and Technology-Hellas (FORTH-IESL), 70013 Heraklion, Greece.
Department of Materials Science and Technology, University of Crete, 70013 Heraklion, Greece.
Polymers (Basel). 2020 Feb 4;12(2):316. doi: 10.3390/polym12020316.
The design and synthesis of new biomaterials with adjustable physicochemical and biological properties for tissue engineering applications have attracted great interest. In this work, chitosan--poly(l-lactide) (CS--PLLA) copolymers were prepared by chemically binding poly(l-lactide) (PLLA) chains along chitosan (CS) via the "" approach to obtain hybrid biomaterials that present enhanced mechanical stability, due to the presence of PLLA, and high bioactivity, conferred by CS. Two graft copolymers were prepared, CS--PLLA(80/20) and CS--PLLA(50/50), containing 82 wt % and 55 wt % CS, respectively. Degradation studies of compressed discs of the copolymers showed that the degradation rate increased with the CS content of the copolymer. Nanomechanical studies in the dry state indicated that the copolymer with the higher CS content had larger Young modulus, reduced modulus and hardness values, whereas the moduli and hardness decreased rapidly following immersion of the copolymer discs in alpha-MEM cell culture medium for 24 h. Finally, the bioactivity of the hybrid copolymers was evaluated in the adhesion and growth of MC3T3-E1 pre-osteoblastic cells. In vitro studies showed that MC3T3-E1 cells exhibited strong adhesion on both CS--PLLA graft copolymer films from the first day in cell culture, whereas the copolymer with the higher PLLA content, CS--PLLA(50/50), supported higher cell growth.
设计和合成具有可调节物理化学和生物学特性的新型生物材料用于组织工程应用已引起了极大的关注。在这项工作中，壳聚糖-聚（L-丙交酯）（CS-PLLA）共聚物是通过“”方法沿着壳聚糖（CS）化学结合聚（L-丙交酯）（PLLA）链制备而成，以获得由于PLLA的存在而具有增强机械稳定性且由CS赋予高生物活性的杂化生物材料。制备了两种接枝共聚物，CS-PLLA(80/20)和CS-PLLA(50/50)，分别含有82 wt%和55 wt%的CS。共聚物压缩盘的降解研究表明，降解速率随共聚物中CS含量的增加而增加。干态下的纳米力学研究表明，CS含量较高的共聚物具有更大的杨氏模量、降低的模量和硬度值，而将共聚物盘浸入α-MEM细胞培养基中24小时后，模量和硬度迅速下降。最后，在MC3T3-E1前成骨细胞的黏附和生长中评估了杂化共聚物的生物活性。体外研究表明，从细胞培养的第一天起，MC3T3-E1细胞在两种CS-PLLA接枝共聚物膜上均表现出强烈的黏附，而PLLA含量较高的共聚物CS-PLLA(50/50)支持更高的细胞生长。
