He Ying, Wang Wei, Tang Xuyan, Liu Xin
Key Lab of Oral Diseases Research of Anhui Province, Stomatologic Hospital & College, Anhui Medical University.
Department of Stomatology, The Second People's Hospital of Wuhu.
Dent Mater J. 2017 May 31;36(3):325-332. doi: 10.4012/dmj.2016-203. Epub 2017 Feb 22.
A novel chitosan/polycaprolactone (CS/PCL) nanofibrous membrane by electrospinning was developed for guided tissue regeneration (GTR) to improve mechanical properties and to promote osteogenic differentiation. Firstly, chitosan and PCL solutions of different weight ratios (0/100, 30/70, 50/50) were mixed and then electrospun. Our data demonstrated that the CS/PCL (30/70) nanofibrous membrane promoted an increased rBMSCs proliferation when compared to the CS/PCL (50/50) membrane and pure PCL (0/100) membrane. The highest ALP activity and extracellular calcium deposit were observed on the CS/PCL (30/70) nanofibrous membrane, followed by the CS/PCL (50/50) and pure PCL nanofibrous membrane. Furthermore, the expression of osteocalcin (OCN) and Runx2 were also significantly higher on the CS/PCL (30/70, 50/50) nanofibrous membrane as compared to the pure PCL nanofibrous membrane. In conclusion, the electrospun CS/PCL nanofibrous membrane was found to be a biocompatible material that could stimulate osteogenic differentiation, suggesting that the novel CS/PCL membrane has an interesting potential as use for GTR.
通过静电纺丝制备了一种新型壳聚糖/聚己内酯（CS/PCL）纳米纤维膜用于引导组织再生（GTR），以改善机械性能并促进成骨分化。首先，将不同重量比（0/100、30/70、50/50）的壳聚糖和PCL溶液混合，然后进行静电纺丝。我们的数据表明，与CS/PCL（50/50）膜和纯PCL（0/100）膜相比，CS/PCL（30/70）纳米纤维膜促进了rBMSCs增殖的增加。在CS/PCL（30/70）纳米纤维膜上观察到最高的碱性磷酸酶（ALP）活性和细胞外钙沉积，其次是CS/PCL（50/50）和纯PCL纳米纤维膜。此外，与纯PCL纳米纤维膜相比，CS/PCL（30/70、50/50）纳米纤维膜上骨钙素（OCN）和Runx2的表达也显著更高。总之，发现静电纺丝的CS/PCL纳米纤维膜是一种可刺激成骨分化的生物相容性材料，这表明新型CS/PCL膜作为GTR用途具有有趣的潜力。
