体内活体生物萤光成像钴在小鼠模型中的积累。
In Vivo Bioluminescence Imaging of Cobalt Accumulation in a Mouse Model.
机构信息
Department of Anaesthesiology & Critical Care Medicine , Translational Neuroscience Center, West China Brain Research Center, West China Hospital, Sichuan University , Chengdu , Sichuan 610041 , China.
Department of Medicinal Chemistry, Key Laboratory of Chemical Biology (MOE), School of Pharmacy , Shandong University , Jinan , Shandong 250012 , China.
出版信息
Anal Chem. 2018 Apr 17;90(8):4946-4950. doi: 10.1021/acs.analchem.8b00391. Epub 2018 Mar 30.
As a trace element nutrient, cobalt is critical for both prokaryotes and eukaryotes. In the current study, a turn-on Cobalt Bioluminescent Probe 1 (CBP-1) for the detection of cobalt has been successfully developed based on oxidative C-O bond cleavage. This probe exhibited high selectivity and sensitivity toward cobalt over other analytes. By using CBP-1, the successful in vivo imaging of cobalt accumulation was carried out in a mouse model. Such an ability to determine cobalt in living animals provides a powerful technology for studying the system distribution, toxic potency, and biological effect of Co.
作为一种微量元素营养物质，钴对于原核生物和真核生物都至关重要。在本研究中，基于氧化 C-O 键断裂，成功开发了一种用于检测钴的钴生物发光探针 1（CBP-1）。该探针对钴具有高选择性和灵敏度，优于其他分析物。通过使用 CBP-1，成功地在小鼠模型中进行了钴积累的体内成像。这种在活体动物中测定钴的能力为研究 Co 的系统分布、毒性和生物学效应提供了一种强大的技术。
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