Nanotube Research Center, National Institute of Advanced Industrial Science and Technology, 1-1-1 Higashi, Tsukuba, Japan.
Nanomedicine. 2013 Jul;9(5):657-64. doi: 10.1016/j.nano.2012.11.011. Epub 2013 Jan 2.
Carbon nanotubules, such as nanotubes and nanohorns, are potentially useful as drug delivery or hyperthermia agents for cancer therapy. However, the biokinetics of variously sized nanocarbons are important for their medical application and risk assessment. To examine the time course of the biodistribution of carbon nanohorns (CNHs) in mice, CNH aggregates of 100nm (L-CNHs) or CNHs of 30-50nm (S-CNHs) were dispersed with lipid polyethylene glycol and administered to mice through tail vein injection. Histological observation revealed that S-CNHs accumulated more slowly than did L-CNHs in the liver and spleen. The accumulation of L- and S-CNHs in spleen reached saturation within 1 and 48h, respectively, and the accumulation in liver reached saturation within 48h and >7days, respectively. CNHs did not accumulate appreciably in the lung, skin, or kidney. Histologic, hematologic, and immunologic (IL-6, TNF-α, and IFN-γ) tests did not reveal obvious toxicologic lesions at any time point.
In this study the biodistribution and accumulation characteristics of small and large carbon nanohorns were characterized in mice. Data demonstrate slower accumulation of small carbon nanohorns in liver and spleen, no accumulation in skin, lung, or kidney, and no obvious hematologic or immunologic toxicity.
碳纳米管，如纳米管和纳米角，作为药物输送或癌症治疗的热疗剂具有潜在的用途。然而，各种大小的纳米碳的生物动力学对于它们的医学应用和风险评估很重要。为了研究碳纳米角（CNHs）在小鼠体内的生物分布时间过程，将 100nm 的 CNH 聚集体（L-CNHs）或 30-50nm 的 CNHs（S-CNHs）与脂质聚乙二醇分散，并通过尾静脉注射将其施用于小鼠。组织学观察表明，S-CNHs 在肝脏和脾脏中的积累速度比 L-CNHs 慢。L-CNHs 和 S-CNHs 在脾脏中的积累分别在 1h 和 48h 内达到饱和，而在肝脏中的积累分别在 48h 和>7d 内达到饱和。CNHs 在肺部、皮肤或肾脏中没有明显积累。组织学、血液学和免疫学（IL-6、TNF-α 和 IFN-γ）测试在任何时间点均未显示明显的毒性病变。
在这项研究中，研究了小尺寸和大尺寸碳纳米角在小鼠中的生物分布和积累特征。数据表明，小尺寸碳纳米角在肝脏和脾脏中的积累速度较慢，在皮肤、肺或肾脏中没有积累，并且没有明显的血液学或免疫学毒性。
