Laboratório de Oncobiologia Molecular (LabOMol), Departamento de Biotecnologia Farmacêutica, Faculdade de Farmácia, Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro, RJ, Brazil.
Instituto de Pesquisa em Produtos Naturais (IPPN), Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro, RJ, 21941-903, Brazil.
J Bioenerg Biomembr. 2018 Apr;50(2):93-105. doi: 10.1007/s10863-018-9750-3. Epub 2018 Mar 28.
Breast cancer is the major cause of death by cancer in women worldwide and in spite of the many drugs for its treatment, there is still the need for novel therapies for its control. Ocimum species have been used by traditional medicine to control several diseases, including cancer. We have previously characterized the antidiabetic properties of the unfractionated aqueous leaf extracts of Ocimum basilicum (OB) and Ocimum gratissimum (OG), modulating glucose metabolism in diabetic mice. Since glucose metabolism is primordial for cancer cells survival, we hypothesized that these extracts are effective against cancer cells. The unfractionated aqueous leaf extracts of OB and OG were chemically characterized and tested for their cytotoxic, cytostatic and anti-proliferative properties against the human breast cancer cell line MCF-7. Both extracts presented cytostatic effects with an 80% decrease in MCF-7 cell growth at 1 mg/mL. However, only OB promoted cytotoxic effects, interfering with the cell viability even after interruption of the treatment. Moreover, OB but not OG affected the cell proliferation and metabolism, evaluated in terms of lactate production and intracellular ATP content. After 24 h of treatment, OB treated cells presented an apoptotic profile, while OG treated cells were more necrotic. The treatment with both extracts also activated AMPK, but OB was much more efficient than OG in promoting this. The activation of mTOR signaling, another survival pathway was promoted by OB, whereas OG failed to activate it. In the end, we conclude that OB extract is efficient against the human breast cancer cell line.
乳腺癌是全球女性癌症死亡的主要原因，尽管有许多治疗药物，但仍需要新的疗法来控制它。奥罗莫氏菌属的植物被传统医学用于控制多种疾病，包括癌症。我们之前已经描述了奥罗莫氏菌属（OB）和奥罗莫氏菌属（OG）未分级的水提叶提取物的抗糖尿病特性，调节糖尿病小鼠的葡萄糖代谢。由于葡萄糖代谢是癌细胞生存的首要条件，我们假设这些提取物对癌细胞有效。OB 和 OG 的未分级水提叶提取物进行了化学表征，并测试了它们对人乳腺癌 MCF-7 细胞系的细胞毒性、细胞生长抑制和抗增殖特性。两种提取物均表现出细胞生长抑制作用，在 1mg/mL 时 MCF-7 细胞生长减少 80%。然而，只有 OB 促进了细胞毒性作用，即使中断治疗后，也会干扰细胞活力。此外，OB 而非 OG 影响细胞增殖和代谢，根据乳酸产量和细胞内 ATP 含量进行评估。治疗 24 小时后，OB 处理的细胞呈现凋亡特征，而 OG 处理的细胞则更具坏死性。两种提取物的处理还激活了 AMPK，但 OB 比 OG 更有效地促进了这一点。OB 促进了另一条生存途径 mTOR 信号的激活，而 OG 则未能激活它。最后，我们得出结论，OB 提取物对人乳腺癌细胞系有效。
