Unal Yilmaz, Tuncal Salih, Kosmaz Koray, Kucuk Berkay, Kismet Kemal, Cavusoglu Turgut, Celepli Pinar, Senes Mehmet, Yildiz Selin, Hucumenoglu Sema
a Department of General Surgery , Ankara Education and Research Hospital , Ankara , Turkey.
b Department of Pathology , Ankara Education and Research Hospital , Ankara , Turkey.
J Invest Surg. 2019 Apr;32(3):238-244. doi: 10.1080/08941939.2018.1451936. Epub 2018 Mar 28.
Purpose/Aim of the study: Inflammation and oxidative stress are two significant factors affecting the degree of liver damage in obstructive jaundice. The aim of this study was to evaluate the effect of calcium dobesilate (CaDob), an effective antioxidant and anti-inflammatory drug, on damage to liver caused by experimental obstructive jaundice.
30 rats in total were randomly placed into three groups, each group consisting of 10 rats. The sham group (Group 1) only received solely laparotomy. In the control group (Group 2), ligation was applied to the biliary tract and no treatment was implemented. In the CaDob group (Group 3), following ligation of the biliary tract, 100 mg/kg/day CaDob was implemented via an orogastric tube for a 10-day period. Liver tissue and blood samples were taken for histopathological and biochemical examination.
The CaDob group had significantly lower test values for serum liver functions when compared to the control group. Statistically lower levels of tissue malondialdehyde (MDA) and fluorescent oxidation products (FOP) were detected in the CaDob group, and the CaDob group had significantly higher levels of sulfydryl (SH) than the control group. Histopathological scores in the CaDob group were found out to be statistically less than the scores the control group received (p < 0.05).
CaDob treatment repaired the histpatological changes induced by bile duct ligation. The hepatoprotective effects of CaDob can be associated with its antioxidant properties of the drug.
研究目的：炎症和氧化应激是影响梗阻性黄疸肝损伤程度的两个重要因素。本研究的目的是评估多贝斯钙（CaDob），一种有效的抗氧化和抗炎药物，对实验性梗阻性黄疸所致肝损伤的影响。
总共30只大鼠随机分为三组，每组10只。假手术组（第1组）仅接受单纯剖腹手术。对照组（第2组）对胆道进行结扎且不进行治疗。在CaDob组（第3组）中，在结扎胆道后，通过胃管给予100mg/kg/天的CaDob，持续10天。采集肝组织和血液样本进行组织病理学和生化检查。
与对照组相比，CaDob组血清肝功能检测值显著更低。在CaDob组中检测到组织丙二醛（MDA）和荧光氧化产物（FOP）水平在统计学上更低，并且CaDob组的巯基（SH）水平显著高于对照组。发现CaDob组的组织病理学评分在统计学上低于对照组（p<0.05）。
CaDob治疗修复了胆管结扎引起的组织病理学变化。CaDob的肝保护作用可能与其药物的抗氧化特性有关。
