Department of Biomedical Engineering, Faculty of Engineering, National University of Singapore, Singapore, 117583, Singapore.
NUS Graduate School for Integrative Sciences and Engineering, National University of Singapore, Singapore, 117456, Singapore.
Nat Commun. 2018 Mar 28;9(1):1254. doi: 10.1038/s41467-018-03596-z.
Disease diagnostics requires detection and quantification of nano-sized bioparticles including DNA, proteins, viruses, and exosomes. Here, a fluorescent label-free method for sensitive detection of bioparticles is explored using a pillar array with micrometer-sized features in a deterministic lateral displacement (DLD) device. The method relies on measuring changes in size and/or electrostatic charges of 1 µm polymer beads due to the capture of target bioparticles on the surface. These changes can be sensitively detected through the lateral displacement of the beads in the DLD array, wherein the lateral shifts in the output translates to a quantitative measurement of bioparticles bound to the bead. The detection of albumin protein and nano-sized polymer vesicles with a concentration as low as 10 ng mL (150 pM) and 3.75 μg mL, respectively, is demonstrated. This label-free method holds potential for point-of-care diagnostics, as it is low-cost, fast, sensitive, and only requires a standard laboratory microscope for detection.
疾病诊断需要检测和定量分析纳米级生物粒子，包括 DNA、蛋白质、病毒和外泌体。在这里，我们探索了一种使用具有微尺寸特征的柱状阵列的无荧光标记方法，在确定性侧向位移（DLD）装置中用于生物粒子的灵敏检测。该方法依赖于测量由于目标生物粒子在表面上的捕获而导致的 1 μm 聚合物珠的尺寸和/或静电荷的变化。这些变化可以通过在 DLD 阵列中珠的侧向位移来灵敏地检测，其中输出的侧向位移转化为与珠结合的生物粒子的定量测量。分别以低至 10ng/mL（150pM）和 3.75μg/mL 的浓度，演示了对白蛋白蛋白和纳米级聚合物囊泡的检测。这种无标记方法具有用于即时诊断的潜力，因为它成本低、速度快、灵敏度高，并且仅需要标准实验室显微镜进行检测。
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