Kappler Matthias, Kotrba Johanna, Kaune Tom, Bache Matthias, Rot Swetlana, Bethmann Daniel, Wichmann Henri, Güttler Antje, Bilkenroth Udo, Horter Susanne, Gallwitz Lisa, Kessler Jacqueline, Greither Thomas, Taubert Helge, Eckert Alexander W, Vordermark Dirk
Department of Oral and Maxillofacial Plastic Surgery, Martin Luther University Halle-Wittenberg, Halle (Saale), Germany.
Department of Radiotherapy, Martin Luther University Halle-Wittenberg, Halle (Saale), Germany.
Clin Transl Radiat Oncol. 2017 Jun 27;5:6-11. doi: 10.1016/j.ctro.2017.05.002. eCollection 2017 Aug.
Hypoxia gene expression signatures are of high prognostic value for head and neck cancer patients. Recently, the prognostic information of a multiple-gene hypoxia signature was found to be provided by the mRNA level of alone (Tawk et al., 2016). Therefore, we studied the prognostic value of in an independent cohort of oral squamous cell carcinoma (OSCC) patients.
Frozen tumor samples of 118 adult OSCC patients were analysed for mRNA level by quantitative real-time TaqMan™ PCR analysis. Kaplan-Meier analysis and Cox's regression analysis were performed to characterize the prognostic impact of mRNA level in OSCC patients.
The analyzed patient cohort was divided into four subgroups according to the quartiles of the mRNA levels. The highest intratumoral mRNA level was significantly correlated with a poor overall survival (RR = 2.2; = 0.04) and an increased risk of locoregional recurrence (RR = 4.8; = 0.02). In patients who received radiotherapy ( = 82) highest intratumoral mRNA level was significantly correlated with a poor overall survival (RR = 3.4; = 0.01) and an increased risk of locoregional recurrence (RR = 10.3; = 0.005). Moreover, significant correlations between the mRNA level and the mRNA level of several EMT and stem cell markers were found.
A high mRNA level, as a single-gene surrogate of hypoxia, is an independent prognostic marker for the overall survival and locoregional recurrence of OSCC patients.
缺氧基因表达特征对头颈部癌患者具有很高的预后价值。最近发现，单独的mRNA水平就能提供多基因缺氧特征的预后信息（Tawk等人，2016年）。因此，我们在一组独立的口腔鳞状细胞癌（OSCC）患者中研究了其预后价值。
通过定量实时TaqMan™ PCR分析，对118例成年OSCC患者的冷冻肿瘤样本进行mRNA水平分析。采用Kaplan-Meier分析和Cox回归分析来描述mRNA水平对OSCC患者的预后影响。
根据mRNA水平的四分位数，将分析的患者队列分为四个亚组。肿瘤内最高的mRNA水平与较差的总生存率显著相关（RR = 2.2；= 0.04），且局部区域复发风险增加（RR = 4.8；= 0.02）。在接受放疗的患者（= 82）中，肿瘤内最高的mRNA水平与较差的总生存率显著相关（RR = 3.4；= 0.01），且局部区域复发风险增加（RR = 10.3；= 0.005）。此外，还发现mRNA水平与几种EMT和干细胞标志物的mRNA水平之间存在显著相关性。
高mRNA水平作为缺氧的单基因替代指标，是OSCC患者总生存和局部区域复发的独立预后标志物。
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