Key Laboratory of Marine Environmental Corrosion and Bio-fouling, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071, China.
Open Studio for Marine Corrosion and Protection, Qingdao National Laboratory for Marine Science and Technology, 1 Wenhai Road, Qingdao, 266237, China.
Mikrochim Acta. 2017 Dec 1;185(1):1. doi: 10.1007/s00604-017-2562-z.
Single phase metastable α-AgVO microrods with high crystallinity, tetragonal rod-like microstructure, uniform particle size distribution, and good dispersion were synthesized by direct coprecipitation at room temperature. They are shown to be viable peroxidase mimics that catalyze the oxidation of 3,3',5,5'-tetramethylbenzidine in the presence of HO. Kinetic analysis indicated typical Michaelis-Menten catalytic behavior. The findings were used to design a colorimetric assay for HO, best measured at 652 nm. The method has a linear response in the 60 to 200 μM HO concentration range, with a 2 μM detection limit. Benefitting from the chemical stability of the microrods, the method is well reproducible. It also is easily performed and highly specific. Graphic abstract Single phase metastable α-AgVO microrods with high crystallinity, tetragonal rod-like microstructure, uniform particle size distribution, and good dispersion can efficiently catalyze the oxidation reaction of peroxidase substrate 3,3',5,5'-tetramethylbenzidine (TMB) in the presence of HO to produce a blue color change.
室温下直接共沉淀法合成了具有高结晶度、四方棒状微结构、均匀粒径分布和良好分散性的单相亚稳α-AgVO 微米棒。它们被证明是可行的过氧化物酶模拟物，能够在 HO 的存在下催化 3,3',5,5'-四甲基联苯胺的氧化。动力学分析表明其具有典型的米氏酶催化行为。该发现用于设计 HO 的比色测定法，最佳测量波长为 652nm。该方法在 60 至 200 μM HO 浓度范围内具有线性响应，检测限为 2 μM。受益于微米棒的化学稳定性，该方法具有良好的重现性。它还易于操作且具有高度特异性。
