Shanghai Key Laboratory of Chemical Assessment and Sustainability, Department of Chemistry, College of Environmental Science and Technology, Tongji University, Shanghai 200092, China.
Shanghai Key Laboratory of Chemical Assessment and Sustainability, Department of Chemistry, College of Environmental Science and Technology, Tongji University, Shanghai 200092, China.
Biosens Bioelectron. 2016 Dec 15;86:432-438. doi: 10.1016/j.bios.2016.06.036. Epub 2016 Jun 14.
A label-free sensing strategy based on the enzyme-mimicking activity of MOF was demonstrated for colorimetric detection of biomolecules. Firstly obvious blue color was observed due to the high efficiency of peroxidase-like catalytic activity of Fe-MIL-88A (an ion-based MOF material) toward 3,3',5,5'-tetramethylbenzidine (TMB). Then in the presence of target biomolecule and corresponding aptamer, the mimetic activity of Fe-MIL-88A can be strongly inhibited and used directly to realize the colorimetric detection. On the basis of the interesting findings, we designed a straightforward, label-free and sensitive colorimetric method for biomolecule detection by using the enzyme mimetic property of MOF coupling with molecular recognition element. Compared with the existed publications, our work breaks the routine way by setting up an inorganic-organic MOF-aptamer hybrid platform for colorimetric determination of biomolecules, expanding the targets scope from H2O2 or glucose to biomolecules. As a proof of concept, thrombin and thrombin aptamer was used as a model analyte. The limit of detection of 10nM can be achieved with naked eyes and ultrahigh selectivity of thrombin toward numerous interfering substances with 10-fold concentration was demonstrated significantly. Of note, the method was further applied for the detection of thrombin in human serum samples, showing the results in agreement with those values obtained in an immobilization buffer by the colorimetric method. This inorganic-organic MOF-aptamer sensing strategy may in principle be universally applicable for the detection of a range of environmental or biomedical molecules of interests.
基于 MOF 的酶模拟活性，展示了一种无标记传感策略，用于生物分子的比色检测。首先，由于 Fe-MIL-88A（一种基于离子的 MOF 材料）对 3,3',5,5'-四甲基联苯胺（TMB）具有高效的过氧化物酶样催化活性，观察到明显的蓝色。然后，在存在靶生物分子和相应适配体的情况下，Fe-MIL-88A 的模拟活性可以被强烈抑制，并直接用于比色检测。基于这一有趣的发现，我们设计了一种简单、无标记和灵敏的比色法，用于通过 MOF 的酶模拟特性与分子识别元件结合来检测生物分子。与已有的出版物相比，我们的工作通过建立无机-有机 MOF-适配体杂化平台用于生物分子的比色测定，打破了常规方法，将目标范围从 H2O2 或葡萄糖扩展到生物分子。作为概念验证，凝血酶和凝血酶适配体被用作模型分析物。可以用肉眼检测到 10nM 的检测限，并且显著证明了凝血酶对 10 倍浓度的大量干扰物质的超高选择性。值得注意的是，该方法进一步用于人血清样品中凝血酶的检测，显示出与比色法在固定化缓冲液中获得的结果一致。这种无机-有机 MOF-适配体传感策略原则上可以普遍适用于一系列环境或生物医学感兴趣分子的检测。
