Division of Biosciences, Research Department of Structural and Molecular Biology, University College London, Darwin Building, Gower Street, London, WC1E 6BT, UK.
Department of Chemistry, Biochemistry and Environmental Protection, Faculty of Sciences, University of Novi Sad, Trg Dositeja Obradovića 3, Novi Sad, 21000, Serbia.
Eur J Nutr. 2019 Mar;58(2):743-753. doi: 10.1007/s00394-018-1680-7. Epub 2018 Mar 28.
There is general agreement that some dietary polyphenols block non-haem iron uptake, but the mechanisms by which they achieve this action are poorly understood. Since the polyphenol quercetin is ingested daily in significant amounts, we have investigated the effect of quercetin on duodenal non-haem iron absorption in vivo, as well as its effect on factors known to be involved in systemic iron metabolism.
Rats were subject to gastric gavage and systemic quercetin administration. Treatments were followed with uptake studies using radiolabeled iron, serum iron and transferrin saturation measurements, LC-MS/MS analysis of quercetin metabolites in serum, determination of tissue non-haem iron content and analysis of gene expression of iron-related proteins.
Both oral and intraperitoneal (IP) quercetin caused serum and tissue iron depletion by two means, first by increasing mucosal iron uptake and inhibiting iron efflux from duodenal mucosa, and second by decreasing levels of duodenal DMT1, Dcytb and FPN. Additionally, IP quercetin induced highly significant increased liver expression of hepcidin, a hormone known to inhibit intestinal iron uptake.
Oral quercetin significantly inhibited iron absorption, while IP quercetin significantly affected iron-related genes. These results could lead to development of new effective ways of preventing and treating iron deficiency anaemia, the most widespread nutritional disorder in the world.
人们普遍认为，一些膳食多酚会阻止非血红素铁的吸收，但它们实现这一作用的机制还知之甚少。由于多酚槲皮素每天都以大量的形式被摄入，我们研究了槲皮素对体内十二指肠非血红素铁吸收的影响，以及它对已知参与全身铁代谢的因素的影响。
对大鼠进行胃灌胃和全身槲皮素给药。用放射性标记铁、血清铁和转铁蛋白饱和度测量、血清中槲皮素代谢物的 LC-MS/MS 分析、组织非血红素铁含量测定和铁相关蛋白基因表达分析来跟踪处理。
口服和腹腔内（IP）槲皮素通过两种方式导致血清和组织铁耗竭，首先是通过增加黏膜铁摄取并抑制十二指肠黏膜铁外排，其次是通过降低十二指肠 DMT1、Dcytb 和 FPN 的水平。此外，IP 槲皮素诱导肝脏中与铁相关的基因明显高表达，这是一种已知抑制肠道铁吸收的激素。
口服槲皮素显著抑制铁吸收，而 IP 槲皮素显著影响铁相关基因。这些结果可能为开发预防和治疗缺铁性贫血的新方法提供依据，缺铁性贫血是世界上最普遍的营养障碍。
