Huang Yutong, Zhang Simiao, Zhou Mei, Xu Xiaokang, Sun Weiqing, Ma Jing, Wu Long
College of Life Science, Yangtze University, Jingzhou 434023, China.
School of Food Science and Engineering, Key Laboratory of Tropical and Vegetables Quality and Safety for State Market Regulation, Hainan University, Haikou 570228, China.
Int J Mol Sci. 2024 Dec 27;26(1):139. doi: 10.3390/ijms26010139.
Biogenic amines (BAs), produced in fish and seafood due to microbial contamination, pose significant health risks. This study introduces a novel ratiometric fluorescent probe, synthesized by integrating rhodamine 6G(R6G) and gold nanoparticles (AuNCs), for the sensitive and specific detection of BAs. The probe operates on the principle of BAs hydrolysis, catalyzed by diamine oxidase, to produce hydrogen peroxide (HO), which selectively quenches the fluorescence of AuNCs at 620 nm, while the fluorescence of R6Gat 533 nm remains unaffected. The ratio of I/I demonstrated excellent linearity with a wide dynamic range (1-1000 μM) and a low detection limit of 0.1 μM. Validation using grass carp samples showed high recovery rates (97.57% to 104.29%), confirming the probe's efficacy and potential for practical application in food safety monitoring.
由于微生物污染，鱼类和海鲜中产生的生物胺（BAs）会带来重大健康风险。本研究介绍了一种新型比率荧光探针，它通过整合罗丹明6G（R6G）和金纳米颗粒（AuNCs）合成，用于灵敏且特异性地检测生物胺。该探针基于生物胺水解原理工作，在二胺氧化酶催化下产生过氧化氢（HO），过氧化氢能选择性猝灭金纳米颗粒在620nm处的荧光，而罗丹明6G在533nm处的荧光不受影响。I/I的比率在宽动态范围（1 - 1000μM）内表现出优异的线性，检测限低至0.1μM。使用草鱼样本进行的验证显示回收率很高（97.57%至104.29%），证实了该探针在食品安全监测实际应用中的有效性和潜力。
