University of Rochester, Department of Biomedical Engineering, Rochester, New York, United States.
University of Rochester, Institute of Optics, Rochester, New York, United States.
J Biomed Opt. 2018 Mar;23(3):1-9. doi: 10.1117/1.JBO.23.3.035006.
Nicotinamide has been shown to affect blood flow in both tumor and normal tissues, including skeletal muscle. Intraperitoneal injection of nicotinamide was used as a simple intervention to test the sensitivity of noninvasive diffuse correlation spectroscopy (DCS) to changes in blood flow in the murine left quadriceps femoris skeletal muscle. DCS was then compared with the gold-standard fluorescent microsphere (FM) technique for validation. The nicotinamide dose-response experiment showed that relative blood flow measured by DCS increased following treatment with 500- and 1000-mg / kg nicotinamide. The DCS and FM technique comparison showed that blood flow index measured by DCS was correlated with FM counts quantified by image analysis. The results of this study show that DCS is sensitive to nicotinamide-induced blood flow elevation in the murine left quadriceps femoris. Additionally, the results of the comparison were consistent with similar studies in higher-order animal models, suggesting that mouse models can be effectively employed to investigate the utility of DCS for various blood flow measurement applications.
烟酰胺已被证明可影响肿瘤和正常组织（包括骨骼肌）中的血流。本研究采用腹腔内注射烟酰胺作为一种简单的干预措施，以测试非侵入性漫反射相关光谱（DCS）技术对小鼠左股四头肌血流变化的敏感性，并与荧光微球（FM）技术进行了比较以验证其准确性。烟酰胺剂量反应实验表明，用 500-和 1000-mg/kg 烟酰胺处理后，DCS 测量的相对血流增加。DCS 和 FM 技术比较表明，DCS 测量的血流指数与通过图像分析量化的 FM 计数相关。这项研究的结果表明，DCS 对小鼠左股四头肌中烟酰胺诱导的血流升高敏感。此外，比较结果与更高阶动物模型中的类似研究一致，这表明可以有效地使用小鼠模型来研究 DCS 在各种血流测量应用中的实用性。
