Metallothionein in blood, bile and urine.

The development of radioimmunoassays for rat metallothionein-I (MT-I) has revealed that this protein is present in many extracellular fluids. Plasma concentrations are low in normal animals but may be substantially increased in animals with elevated tissue MT levels. MT-I is also excreted in varying amounts into urine and bile, and there is evidence for the presence of degradation products of MT-I, detectable by radioimmunoassay, in these fluids. Investigation of the secretion of MT can provide valuable information on the turnover of the protein in tissues and lead to the development of novel diagnostic tests for the assessment of trace metal status.