注射铜和锌的大鼠体内金属硫蛋白及其可能的降解产物的胆汁排泄
Biliary excretion of metallothionein and a possible degradation product in rats injected with copper and zinc.
作者信息
Sato M, Bremner I
出版信息
Biochem J. 1984 Oct 15;223(2):475-9. doi: 10.1042/bj2230475.
Abstract
The concentrations of metallothionein-I (MT-I) and related immunoreactive products in bile from adult female rats were measured by radioimmunoassay. Concentrations in normal animals were 20-30 ng/ml, but increased to 600 and 75 ng/ml after injection of Cu2+ and Zn2+ respectively (3 mg of metal/kg body wt.). However, only 1-2% of the biliary Cu was bound to MT, and less than 1% of the total liver MT in control or Cu2+-injected rats appeared to be secreted in intact form into bile. Other major immunoreactive components in bile from Cu2+-injected rats included an aggregated form of MT-I and a possible degradation product of the isoprotein.
摘要
通过放射免疫分析法测定成年雌性大鼠胆汁中金属硫蛋白-I（MT-I）及其相关免疫反应性产物的浓度。正常动物胆汁中的浓度为20 - 30纳克/毫升，但分别注射铜离子（Cu2+）和锌离子（Zn2+）（3毫克金属/千克体重）后，浓度分别增加到600和75纳克/毫升。然而，胆汁中的铜只有1 - 2%与MT结合，在对照或注射铜离子的大鼠中，肝脏中总MT的不到1%似乎以完整形式分泌到胆汁中。注射铜离子的大鼠胆汁中的其他主要免疫反应性成分包括MT-I的聚集形式和一种可能的同工蛋白降解产物。
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